Detailed information of TA system TA239435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2782369..2783108
• Replicon: chromosome
• Accession: NZ_CP094272
• Organism: Klebsiella aerogenes strain NY1688

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: MQE04_RS13575
• Protein ID: WP_032705770.1
• Coordinates: 2782623..2783108 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: MQE04_RS13570
• Protein ID: WP_015705366.1
• Coordinates: 2782369..2782635 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE04_RS13550 (MQE04_13550)	2778661..2779278	+	618	WP_047058775.1	response regulator	-
MQE04_RS13555 (MQE04_13555)	2779374..2779871	+	498	WP_047080778.1	heme-binding protein	-
MQE04_RS13560 (MQE04_13560)	2780276..2780806	+	531	WP_047058778.1	N-acetyltransferase	-
MQE04_RS13565 (MQE04_13565)	2780861..2782096	-	1236	WP_020078885.1	MFS transporter	-
MQE04_RS13570 (MQE04_13570)	2782369..2782635	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
MQE04_RS13575 (MQE04_13575)	2782623..2783108	+	486	WP_032705770.1	GNAT family N-acetyltransferase	Toxin
MQE04_RS13580 (MQE04_13580)	2783180..2784796	+	1617	WP_042897505.1	FAD-NAD(P)-binding protein	-
MQE04_RS13585 (MQE04_13585)	2784915..2786015	+	1101	WP_129242673.1	NADH:flavin oxidoreductase/NADH oxidase	-
MQE04_RS13590 (MQE04_13590)	2786072..2786230	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
MQE04_RS13595 (MQE04_13595)	2786491..2787561	+	1071	WP_015705361.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17573.23 Da
• Isoelectric Point: 8.8793

>T239435 WP_032705770.1 NZ_CP094272:2782623-2783108 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEESHQVVGFYSLATGSVNHSEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T239435 NZ_LR890714:c4468980-4468771 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT239435 WP_015705366.1 NZ_CP094272:2782369-2782635 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT239435 NZ_LR890714:c4468774-4468472 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9