Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 150184..150709 | Replicon | plasmid pSE228A |
| Accession | NZ_CP094270 | ||
| Organism | Salmonella enterica subsp. enterica serovar Enteritidis strain JL228 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | I3W3D5 |
| Locus tag | MPK88_RS23880 | Protein ID | WP_001159863.1 |
| Coordinates | 150404..150709 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | M7S5D0 |
| Locus tag | MPK88_RS23875 | Protein ID | WP_000813641.1 |
| Coordinates | 150184..150402 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MPK88_RS23840 (MPK88_23805) | 145268..145441 | - | 174 | WP_272487348.1 | hypothetical protein | - |
| MPK88_RS23845 (MPK88_23810) | 145447..146151 | - | 705 | WP_001067858.1 | IS6-like element IS26 family transposase | - |
| MPK88_RS23850 (MPK88_23815) | 146237..146356 | + | 120 | Protein_151 | recombinase | - |
| MPK88_RS23855 (MPK88_23820) | 146725..147714 | - | 990 | WP_000461382.1 | RepB family plasmid replication initiator protein | - |
| MPK88_RS23860 (MPK88_23825) | 148208..148503 | - | 296 | Protein_153 | cytoplasmic protein | - |
| MPK88_RS23865 (MPK88_23830) | 148515..148943 | + | 429 | Protein_154 | hypothetical protein | - |
| MPK88_RS23870 (MPK88_23835) | 148987..149508 | - | 522 | WP_077681952.1 | hypothetical protein | - |
| MPK88_RS23875 (MPK88_23840) | 150184..150402 | + | 219 | WP_000813641.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| MPK88_RS23880 (MPK88_23845) | 150404..150709 | + | 306 | WP_001159863.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| MPK88_RS23885 (MPK88_23850) | 150711..151001 | + | 291 | WP_001266176.1 | hypothetical protein | - |
| MPK88_RS23890 (MPK88_23855) | 150998..151519 | + | 522 | WP_000198608.1 | hypothetical protein | - |
| MPK88_RS23895 (MPK88_23860) | 151554..152336 | + | 783 | WP_000082169.1 | site-specific integrase | - |
| MPK88_RS23900 (MPK88_23865) | 152345..153058 | + | 714 | WP_000545756.1 | EAL domain-containing protein | - |
| MPK88_RS23905 (MPK88_23870) | 153083..153571 | + | 489 | WP_001075507.1 | CaiF/GrlA family transcriptional regulator | - |
| MPK88_RS23910 (MPK88_23875) | 153565..154050 | + | 486 | WP_000905606.1 | membrane protein | - |
| MPK88_RS23915 (MPK88_23880) | 154327..154614 | - | 288 | WP_071530243.1 | hypothetical protein | - |
| MPK88_RS23920 (MPK88_23885) | 154770..155330 | + | 561 | WP_000900095.1 | inverse autotransporter beta domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sul1 / qacE / ere(A) / aac(6')-IIc | fdeC / spvC / spvB | 1..211429 | 211429 | |
| - | flank | IS/Tn | - | - | 145447..146151 | 704 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11587.48 Da Isoelectric Point: 6.9787
>T239421 WP_001159863.1 NZ_CP094270:150404-150709 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSEKVPRDLYPVMHIGDEPYRLLTTDMTSVPATVIGEEV
ADLSLRENDIKNAINLMFRGI
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSEKVPRDLYPVMHIGDEPYRLLTTDMTSVPATVIGEEV
ADLSLRENDIKNAINLMFRGI
Download Length: 306 bp
>T239421 NZ_LR890714:1075740-1076147 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8314.16 Da Isoelectric Point: 4.5203
>AT239421 WP_000813641.1 NZ_CP094270:150184-150402 [Salmonella enterica subsp. enterica serovar Enteritidis]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADDNRNW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADDNRNW
Download Length: 219 bp
>AT239421 NZ_LR890714:1075493-1075759 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | I3W3D5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A656ICA6 |