Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 55961..57584 Replicon plasmid pSE228A
Accession NZ_CP094270
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain JL228

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag MPK88_RS23400 Protein ID WP_000088046.1
Coordinates 55961..57286 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag MPK88_RS23405 Protein ID WP_024136327.1
Coordinates 57291..57584 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPK88_RS23360 (MPK88_23330) 50982..51338 + 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -
MPK88_RS23365 (MPK88_23335) 51680..51883 - 204 WP_001194555.1 hypothetical protein -
MPK88_RS23370 (MPK88_23340) 51945..52802 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
MPK88_RS23375 (MPK88_23345) 52818..53126 - 309 WP_001224687.1 hypothetical protein -
MPK88_RS23380 (MPK88_23350) 53675..54100 - 426 WP_001043843.1 hypothetical protein -
MPK88_RS23385 (MPK88_23355) 54354..55169 - 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
MPK88_RS23390 (MPK88_23360) 55182..55592 - 411 WP_000985911.1 hypothetical protein -
MPK88_RS23395 (MPK88_23365) 55694..55900 - 207 WP_000134171.1 hypothetical protein -
MPK88_RS23400 (MPK88_23370) 55961..57286 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
MPK88_RS23405 (MPK88_23375) 57291..57584 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
MPK88_RS23410 (MPK88_23380) 58188..58580 - 393 WP_000341066.1 hypothetical protein -
MPK88_RS23415 (MPK88_23385) 58953..59294 + 342 WP_001151575.1 hypothetical protein -
MPK88_RS23420 (MPK88_23390) 59386..59577 + 192 WP_000398480.1 hypothetical protein -
MPK88_RS23425 (MPK88_23395) 59627..59908 + 282 WP_000077926.1 hypothetical protein -
MPK88_RS23430 60249..60380 - 132 WP_000374059.1 hypothetical protein -
MPK88_RS23435 (MPK88_23400) 60471..61712 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ere(A) / aac(6')-IIc fdeC / spvC / spvB 1..211429 211429


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T239420 WP_000088046.1 NZ_CP094270:c57286-55961 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T239420 NZ_LR890714:c952809-952435 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT239420 WP_024136327.1 NZ_CP094270:c57584-57291 [Salmonella enterica subsp. enterica serovar Enteritidis]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT239420 NZ_LR890714:c953230-952856 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCAGATGCATACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References