Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4216983..4217764 Replicon chromosome
Accession NZ_CP094269
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain JL228

Toxin (Protein)


Gene name TacT2 Uniprot ID B5R980
Locus tag MPK88_RS20780 Protein ID WP_000626100.1
Coordinates 4216983..4217474 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B5R979
Locus tag MPK88_RS20785 Protein ID WP_001110452.1
Coordinates 4217471..4217764 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPK88_RS20745 (4212443) 4212443..4212790 + 348 WP_000887832.1 divalent cation tolerance protein CutA -
MPK88_RS20750 (4212766) 4212766..4214469 + 1704 WP_000068885.1 protein-disulfide reductase DsbD -
MPK88_RS20755 (4214506) 4214506..4215081 + 576 WP_001188509.1 transcriptional regulator -
MPK88_RS20765 (4215352) 4215352..4215426 - 75 Protein_4057 helix-turn-helix domain-containing protein -
MPK88_RS20770 (4215806) 4215806..4215883 + 78 Protein_4058 porin family protein -
MPK88_RS20775 (4215983) 4215983..4216735 + 753 WP_000842433.1 non-specific acid phosphatase -
MPK88_RS20780 (4216983) 4216983..4217474 - 492 WP_000626100.1 GNAT family N-acetyltransferase Toxin
MPK88_RS20785 (4217471) 4217471..4217764 - 294 WP_001110452.1 DUF1778 domain-containing protein Antitoxin
MPK88_RS20790 (4218081) 4218081..4218302 + 222 WP_001576552.1 hypothetical protein -
MPK88_RS20795 (4218568) 4218568..4219443 + 876 WP_000921676.1 AraC family transcriptional regulator -
MPK88_RS20800 (4219440) 4219440..4219727 + 288 WP_001541332.1 transcriptional regulator RtsB -
MPK88_RS20805 (4219720) 4219720..4220028 - 309 WP_072095651.1 ABC transporter ATP-binding protein -
MPK88_RS20810 (4220027) 4220027..4220275 + 249 Protein_4066 Ig-like domain-containing protein -
MPK88_RS20815 (4220387) 4220387..4220518 + 132 Protein_4067 hypothetical protein -
MPK88_RS20820 (4220812) 4220812..4221717 - 906 WP_001268200.1 YjiK family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(85-145)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17645.45 Da        Isoelectric Point: 7.7297

>T239417 WP_000626100.1 NZ_CP094269:c4217474-4216983 [Salmonella enterica subsp. enterica serovar Enteritidis]
MISTPEPLHAGHILTPFCCGVDSIDNWLKQRAMKNQTTGASRTFVCCGSDSNVLAYYSLASSAVTTNTSPGRFRRNMPDP
IPVVVLGRLAVDKSLHGQGVARALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYQRVGFVPSPMDPMMLMVTLGDLV
ESV

Download         Length: 492 bp

>T239417 NZ_LR890714:262313-262420 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10977.61 Da        Isoelectric Point: 8.6141

>AT239417 WP_001110452.1 NZ_CP094269:c4217764-4217471 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLVRLDQTPSPN
AALRKTMQTPAPWEQEK

Download         Length: 294 bp

>AT239417 NZ_LR890714:c262264-262201 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656IQ80

Antitoxin

Source ID Structure
PDB 7AK7
AlphaFold DB A0A5I1DGA4

References