Detailed information of TA system TA239391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 3097..3681
• Replicon: plasmid pBDT201-1
• Accession: NZ_CP094259
• Organism: Acinetobacter indicus strain BDT201

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: MOW14_RS14275
• Protein ID: WP_000286964.1
• Coordinates: 3097..3399 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MOW14_RS14280
• Protein ID: WP_000985609.1
• Coordinates: 3400..3681 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOW14_RS14250 (MOW14_14250)	1..936	+	936	WP_001292329.1	replication initiation protein RepM	-
MOW14_RS14255 (MOW14_14255)	1014..1532	+	519	WP_242898272.1	plasmid replication DNA-binding protein	-
MOW14_RS14260 (MOW14_14260)	1736..2095	-	360	WP_224223039.1	DUF4829 domain-containing protein	-
MOW14_RS14265 (MOW14_14265)	2194..2487	-	294	WP_227505690.1	hypothetical protein	-
MOW14_RS14270 (MOW14_14270)	2539..2928	-	390	Protein_4	hypothetical protein	-
MOW14_RS14275 (MOW14_14275)	3097..3399	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MOW14_RS14280 (MOW14_14280)	3400..3681	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
MOW14_RS14285 (MOW14_14285)	3782..4081	+	300	WP_000358348.1	hypothetical protein	-
MOW14_RS14290 (MOW14_14290)	4511..4771	-	261	WP_242898273.1	hypothetical protein	-
MOW14_RS14295 (MOW14_14295)	4731..5342	-	612	WP_180009631.1	recombinase family protein	-
MOW14_RS14300 (MOW14_14300)	5527..5916	-	390	WP_032040164.1	tautomerase family protein	-
MOW14_RS14305 (MOW14_14305)	6106..7431	+	1326	WP_242898203.1	ISNCY family transposase	-
MOW14_RS14310 (MOW14_14310)	7579..8211	-	633	WP_104988442.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..14897	14897
	-	flank	IS/Tn	-	-	6094..7431	1337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T239391 WP_000286964.1 NZ_CP094259:3097-3399 [Acinetobacter indicus]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T239391 NZ_LR890702:c2803110-2802700 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATAGCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT239391 WP_000985609.1 NZ_CP094259:3400-3681 [Acinetobacter indicus]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT239391 NZ_LR890702:c2803357-2803091 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94