Detailed information of TA system TA239388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-brnA/BrnT-BrnA
• Location: 8076..8658
• Replicon: plasmid pBDT2076-1
• Accession: NZ_CP094255
• Organism: Acinetobacter indicus strain BDT2076

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: -
• Locus tag: MOW12_RS14985
• Protein ID: WP_034707141.1
• Coordinates: 8371..8658 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: MOW12_RS14980
• Protein ID: WP_180058738.1
• Coordinates: 8076..8384 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOW12_RS14945 (MOW12_14945)	3269..3745	+	477	WP_180058729.1	DNA mismatch endonuclease Vsr	-
MOW12_RS14950 (MOW12_14950)	3768..5087	-	1320	WP_180058728.1	MvaI/BcnI family restriction endonuclease	-
MOW12_RS14955 (MOW12_14955)	5152..6597	-	1446	WP_180058727.1	DNA cytosine methyltransferase	-
MOW12_RS14960 (MOW12_14960)	6737..6879	-	143	Protein_9	helix-turn-helix domain-containing protein	-
MOW12_RS14965 (MOW12_14965)	7051..7335	+	285	WP_180058726.1	hypothetical protein	-
MOW12_RS14970 (MOW12_14970)	7361..7849	-	489	WP_180001825.1	hypothetical protein	-
MOW12_RS14975 (MOW12_14975)	7851..8042	-	192	WP_180001826.1	hypothetical protein	-
MOW12_RS14980 (MOW12_14980)	8076..8384	-	309	WP_180058738.1	BrnA antitoxin family protein	Antitoxin
MOW12_RS14985 (MOW12_14985)	8371..8658	-	288	WP_034707141.1	BrnT family toxin	Toxin
MOW12_RS14990 (MOW12_14990)	8975..9358	+	384	WP_147395704.1	hypothetical protein	-
MOW12_RS14995 (MOW12_14995)	9595..10206	+	612	WP_242912040.1	recombinase family protein	-
MOW12_RS15000 (MOW12_15000)	10631..11158	-	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
MOW12_RS15005 (MOW12_15005)	11447..11695	+	249	WP_180058737.1	hypothetical protein	-
MOW12_RS15010 (MOW12_15010)	11715..12293	-	579	WP_180058736.1	plasmid replication DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..15241	15241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11249.62 Da
• Isoelectric Point: 5.2134

>T239388 WP_034707141.1 NZ_CP094255:c8658-8371 [Acinetobacter indicus]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T239388 NZ_LR890694:104151-104531 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 12060.72 Da
• Isoelectric Point: 9.4855

>AT239388 WP_180058738.1 NZ_CP094255:c8384-8076 [Acinetobacter indicus]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVRDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRNAMIEELKK

Nucleotide

• Length: 309 bp

>AT239388 NZ_LR890694:103930-104151 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA