Detailed information of TA system TA239380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2956611..2957212
• Replicon: chromosome
• Accession: NZ_CP094240
• Organism: Comamonas sp. 7D-2evo2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MP579_RS13695
• Protein ID: WP_019042333.1
• Coordinates: 2956826..2957212 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A076PMR1
• Locus tag: MP579_RS13690
• Protein ID: WP_034356765.1
• Coordinates: 2956611..2956826 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MP579_RS13665 (MP579_13665)	2952052..2952498	+	447	WP_019042328.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
MP579_RS13670 (MP579_13670)	2952578..2952967	-	390	WP_080571250.1	CzcE family metal-binding protein	-
MP579_RS13675 (MP579_13675)	2953219..2954892	+	1674	WP_230629841.1	phosphoethanolamine transferase	-
MP579_RS13680 (MP579_13680)	2955229..2955555	-	327	WP_019042331.1	transposase	-
MP579_RS13685 (MP579_13685)	2955709..2956526	-	818	Protein_2673	SprT-like domain-containing protein	-
MP579_RS13690 (MP579_13690)	2956611..2956826	+	216	WP_034356765.1	DUF2191 domain-containing protein	Antitoxin
MP579_RS13695 (MP579_13695)	2956826..2957212	+	387	WP_019042333.1	twitching motility protein PilT	Toxin
MP579_RS13700 (MP579_13700)	2957301..2957510	+	210	WP_034356767.1	hypothetical protein	-
MP579_RS13705 (MP579_13705)	2957690..2958490	+	801	WP_242881683.1	translesion DNA synthesis-associated protein ImuA	-
MP579_RS13710 (MP579_13710)	2958435..2959745	+	1311	WP_034356769.1	DNA polymerase Y family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2645726..3210757	565031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13999.29 Da
• Isoelectric Point: 5.0594

>T239380 WP_019042333.1 NZ_CP094240:2956826-2957212 [Comamonas sp. 7D-2evo2]
MPVSVLVDTSVWVRHFREADTHLQELLMQDSVLMHSMVHAELACGTPPAPRADTLAALAMLRPSQEASIPETLEFLEQNK
LFGKGCGLVDLSLLASTLITPGALLWTADRRLADMATQLNVAYLPPLH

Nucleotide

• Length: 387 bp

>T239380 NZ_CP094240:2956826-2957212 [Comamonas sp. 7D-2evo2]
ATGCCCGTGTCCGTCCTAGTCGATACATCAGTGTGGGTGAGACATTTTCGTGAGGCAGACACTCACCTGCAGGAATTGCT
TATGCAAGACAGCGTGCTGATGCATTCCATGGTGCATGCAGAGCTGGCCTGTGGTACGCCGCCAGCACCGCGTGCCGACA
CGCTGGCGGCTCTGGCGATGCTGCGGCCGAGCCAGGAGGCATCAATTCCAGAGACTTTGGAATTTCTGGAGCAGAACAAG
CTTTTCGGCAAGGGCTGCGGCTTAGTAGATCTATCGCTGCTTGCCTCTACGCTTATCACTCCTGGCGCCTTACTTTGGAC
TGCAGATCGACGCCTCGCCGACATGGCCACACAGTTGAACGTGGCCTATCTACCGCCTTTGCATTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7519.50 Da
• Isoelectric Point: 5.0166

>AT239380 WP_034356765.1 NZ_CP094240:2956611-2956826 [Comamonas sp. 7D-2evo2]
MRSTFTIDDDVVNRARAVAAPGIAVPELVRLALETFTRVEAGKRLAALGGAAPNMPDVPRRGSEADAEDSR

Nucleotide

• Length: 216 bp

>AT239380 NZ_CP094240:2956611-2956826 [Comamonas sp. 7D-2evo2]
ATGAGATCGACTTTCACGATCGATGACGATGTCGTAAACCGCGCACGCGCCGTCGCGGCACCCGGTATTGCTGTGCCGGA
ACTGGTACGACTCGCACTGGAAACCTTCACACGTGTCGAGGCAGGTAAGCGTTTGGCTGCGCTCGGCGGTGCCGCGCCAA
ATATGCCCGATGTGCCGCGCCGTGGCAGTGAGGCGGATGCTGAGGACTCACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A076PMR1