Detailed information of TA system TA239379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2235946..2236541
• Replicon: chromosome
• Accession: NZ_CP094240
• Organism: Comamonas sp. 7D-2evo2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5D9AQV8
• Locus tag: MP579_RS10305
• Protein ID: WP_034358561.1
• Coordinates: 2235946..2236242 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MP579_RS10310
• Protein ID: WP_187771848.1
• Coordinates: 2236245..2236541 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MP579_RS10285 (MP579_10285)	2230946..2232514	+	1569	WP_034358565.1	sensor domain-containing diguanylate cyclase	-
MP579_RS10290 (MP579_10290)	2233201..2233464	-	264	WP_003061434.1	translation initiation factor IF-1	-
MP579_RS10295 (MP579_10295)	2233488..2233706	-	219	WP_003053604.1	hypothetical protein	-
MP579_RS10300 (MP579_10300)	2234255..2235595	+	1341	WP_034358562.1	integrase family protein	-
MP579_RS10305 (MP579_10305)	2235946..2236242	+	297	WP_034358561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MP579_RS10310 (MP579_10310)	2236245..2236541	+	297	WP_187771848.1	putative addiction module antidote protein	Antitoxin
MP579_RS10315 (MP579_10315)	2236724..2237566	+	843	WP_131324717.1	hypothetical protein	-
MP579_RS10320 (MP579_10320)	2237713..2237961	+	249	WP_034358558.1	hypothetical protein	-
MP579_RS10325 (MP579_10325)	2237961..2238242	+	282	WP_034358555.1	hypothetical protein	-
MP579_RS10330 (MP579_10330)	2238304..2238648	+	345	WP_034358553.1	hypothetical protein	-
MP579_RS10335 (MP579_10335)	2238648..2239598	+	951	WP_052057663.1	toprim domain-containing protein	-
MP579_RS10340 (MP579_10340)	2239588..2241423	+	1836	WP_080746132.1	DUF927 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2228754..2269309	40555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (9-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10954.57 Da
• Isoelectric Point: 6.4592

>T239379 WP_034358561.1 NZ_CP094240:2235946-2236242 [Comamonas sp. 7D-2evo2]
MFTIKRTAEFDAWLFGLRDTMTRIRLAKRLDKVQRGPLGDTKPVGDGVHELREDFGPGWCMYYVQHGDVLVVMLGGGDKS
TQQADIQAAIALSKTLED

Nucleotide

• Length: 297 bp

>T239379 NZ_LR890693:c3997748-3997491 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10576.17 Da
• Isoelectric Point: 5.1006

>AT239379 WP_187771848.1 NZ_CP094240:2236245-2236541 [Comamonas sp. 7D-2evo2]
MTKIADLPTFDMAEHLKTDEDLAAYLSLVLEEDDPAELANALGVIARARGMTEIASASGLTREALYKALRPGSQPRFDTI
RRVCGALGVRLVAQPIHA

Nucleotide

• Length: 297 bp

>AT239379 NZ_LR890693:c3997479-3996928 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A5D9AQV8