Detailed information of TA system TA239377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3931873..3932543
• Replicon: chromosome
• Accession: NZ_CP094239
• Organism: Comamonas sp. 7D-2evo1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: MP576_RS18145
• Protein ID: WP_242881849.1
• Coordinates: 3931873..3932049 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MP576_RS18150
• Protein ID: WP_242881850.1
• Coordinates: 3932115..3932543 (+)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MP576_RS18115 (MP576_18115)	3927789..3928602	+	814	Protein_3542	IS5 family transposase	-
MP576_RS18125 (MP576_18125)	3928996..3929412	+	417	WP_242881844.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
MP576_RS18130 (MP576_18130)	3929417..3930274	+	858	WP_242881846.1	excinuclease Cho	-
MP576_RS18135 (MP576_18135)	3930284..3931582	+	1299	WP_242881847.1	Y-family DNA polymerase	-
MP576_RS18140 (MP576_18140)	3931582..3931794	+	213	WP_242881848.1	hypothetical protein	-
MP576_RS18145 (MP576_18145)	3931873..3932049	+	177	WP_242881849.1	type II toxin-antitoxin system HicA family toxin	Toxin
MP576_RS18150 (MP576_18150)	3932115..3932543	+	429	WP_242881850.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MP576_RS18155 (MP576_18155)	3932556..3933050	-	495	WP_242881851.1	hypothetical protein	-
MP576_RS18160 (MP576_18160)	3933047..3933517	-	471	WP_242881852.1	lysozyme	-
MP576_RS18165 (MP576_18165)	3933514..3933831	-	318	WP_242881853.1	hypothetical protein	-
MP576_RS18170 (MP576_18170)	3933863..3934099	-	237	WP_242881854.1	hypothetical protein	-
MP576_RS18175 (MP576_18175)	3934096..3935991	-	1896	WP_242881855.1	hypothetical protein	-
MP576_RS18180 (MP576_18180)	3935991..3936386	-	396	WP_242881856.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3921992..4010374	88382

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-73)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6737.70 Da
• Isoelectric Point: 11.0738

>T239377 WP_242881849.1 NZ_CP094239:3931873-3932049 [Comamonas sp. 7D-2evo1]
VKISEFKRWLEAQGVKVENGTNHWRLYHNGNTSTLPRHPSKELKEGTRRAILKQLGIN

Nucleotide

• Length: 177 bp

>T239377 NZ_LR890693:3867724-3868074 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15238.48 Da
• Isoelectric Point: 4.8066

>AT239377 WP_242881850.1 NZ_CP094239:3932115-3932543 [Comamonas sp. 7D-2evo1]
MQKYPARFDPADEGGYTVTFRDIPEAITQGDDLAEAESMARDALVTAMEFYFEAGRAVPAPSKAEKGERLVELPASVAVK
VALLNARLATGARPADVARAMGVKPQEMTRVFDLGHATKIDTVAAALAAMGYELELTVRPIK

Nucleotide

• Length: 429 bp

>AT239377 NZ_LR890693:3867479-3867730 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA