Detailed information of TA system TA239371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3479732..3480327
• Replicon: chromosome
• Accession: NZ_CP094238
• Organism: Comamonas sp. 7D-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5D9AQV8
• Locus tag: MPZ60_RS16035
• Protein ID: WP_034358561.1
• Coordinates: 3480031..3480327 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MPZ60_RS16030
• Protein ID: WP_187771848.1
• Coordinates: 3479732..3480028 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPZ60_RS16000 (MPZ60_16000)	3474850..3476685	-	1836	WP_080746132.1	DUF927 domain-containing protein	-
MPZ60_RS16005 (MPZ60_16005)	3476675..3477625	-	951	WP_052057663.1	toprim domain-containing protein	-
MPZ60_RS16010 (MPZ60_16010)	3477625..3477969	-	345	WP_034358553.1	hypothetical protein	-
MPZ60_RS16015 (MPZ60_16015)	3478031..3478312	-	282	WP_034358555.1	hypothetical protein	-
MPZ60_RS16020 (MPZ60_16020)	3478312..3478560	-	249	WP_034358558.1	hypothetical protein	-
MPZ60_RS16025 (MPZ60_16025)	3478707..3479549	-	843	WP_131324717.1	hypothetical protein	-
MPZ60_RS16030 (MPZ60_16030)	3479732..3480028	-	297	WP_187771848.1	putative addiction module antidote protein	Antitoxin
MPZ60_RS16035 (MPZ60_16035)	3480031..3480327	-	297	WP_034358561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MPZ60_RS16040 (MPZ60_16040)	3480678..3482018	-	1341	WP_034358562.1	integrase family protein	-
MPZ60_RS16045 (MPZ60_16045)	3482567..3482785	+	219	WP_003053604.1	hypothetical protein	-
MPZ60_RS16050 (MPZ60_16050)	3482809..3483072	+	264	WP_003061434.1	translation initiation factor IF-1	-
MPZ60_RS16055 (MPZ60_16055)	3483759..3485327	-	1569	WP_034358565.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3453233..3483072	29839

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (9-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10954.57 Da
• Isoelectric Point: 6.4592

>T239371 WP_034358561.1 NZ_CP094238:c3480327-3480031 [Comamonas sp. 7D-2]
MFTIKRTAEFDAWLFGLRDTMTRIRLAKRLDKVQRGPLGDTKPVGDGVHELREDFGPGWCMYYVQHGDVLVVMLGGGDKS
TQQADIQAAIALSKTLED

Nucleotide

• Length: 297 bp

>T239371 NZ_LR890693:2624192-2624656 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10576.17 Da
• Isoelectric Point: 5.1006

>AT239371 WP_187771848.1 NZ_CP094238:c3480028-3479732 [Comamonas sp. 7D-2]
MTKIADLPTFDMAEHLKTDEDLAAYLSLVLEEDDPAELANALGVIARARGMTEIASASGLTREALYKALRPGSQPRFDTI
RRVCGALGVRLVAQPIHA

Nucleotide

• Length: 297 bp

>AT239371 NZ_LR890693:2623857-2624192 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A5D9AQV8