Detailed information of TA system TA239370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2762391..2762992
• Replicon: chromosome
• Accession: NZ_CP094238
• Organism: Comamonas sp. 7D-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MPZ60_RS12660
• Protein ID: WP_019042333.1
• Coordinates: 2762391..2762777 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A076PMR1
• Locus tag: MPZ60_RS12665
• Protein ID: WP_034356765.1
• Coordinates: 2762777..2762992 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPZ60_RS12645 (MPZ60_12645)	2759858..2761168	-	1311	WP_034356769.1	DNA polymerase Y family protein	-
MPZ60_RS12650 (MPZ60_12650)	2761113..2761913	-	801	WP_242881683.1	translesion DNA synthesis-associated protein ImuA	-
MPZ60_RS12655 (MPZ60_12655)	2762093..2762302	-	210	WP_034356767.1	hypothetical protein	-
MPZ60_RS12660 (MPZ60_12660)	2762391..2762777	-	387	WP_019042333.1	twitching motility protein PilT	Toxin
MPZ60_RS12665 (MPZ60_12665)	2762777..2762992	-	216	WP_034356765.1	DUF2191 domain-containing protein	Antitoxin
MPZ60_RS12670 (MPZ60_12670)	2763077..2763894	+	818	Protein_2461	SprT-like domain-containing protein	-
MPZ60_RS12675 (MPZ60_12675)	2764048..2764374	+	327	WP_019042331.1	transposase	-
MPZ60_RS12680 (MPZ60_12680)	2764711..2766384	-	1674	WP_230629841.1	phosphoethanolamine transferase	-
MPZ60_RS12685 (MPZ60_12685)	2766636..2767025	+	390	WP_080571250.1	CzcE family metal-binding protein	-
MPZ60_RS12690 (MPZ60_12690)	2767105..2767551	-	447	WP_019042328.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2517267..2921695	404428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13999.29 Da
• Isoelectric Point: 5.0594

>T239370 WP_019042333.1 NZ_CP094238:c2762777-2762391 [Comamonas sp. 7D-2]
MPVSVLVDTSVWVRHFREADTHLQELLMQDSVLMHSMVHAELACGTPPAPRADTLAALAMLRPSQEASIPETLEFLEQNK
LFGKGCGLVDLSLLASTLITPGALLWTADRRLADMATQLNVAYLPPLH

Nucleotide

• Length: 387 bp

>T239370 NZ_LR890693:c2582099-2581785 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7519.50 Da
• Isoelectric Point: 5.0166

>AT239370 WP_034356765.1 NZ_CP094238:c2762992-2762777 [Comamonas sp. 7D-2]
MRSTFTIDDDVVNRARAVAAPGIAVPELVRLALETFTRVEAGKRLAALGGAAPNMPDVPRRGSEADAEDSR

Nucleotide

• Length: 216 bp

>AT239370 NZ_LR890693:c2581788-2581372 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A076PMR1