Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5334920..5335545 Replicon chromosome
Accession NZ_CP094237
Organism Klebsiella pneumoniae strain K41

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MQE26_RS26015 Protein ID WP_002882817.1
Coordinates 5334920..5335303 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MQE26_RS26020 Protein ID WP_004150355.1
Coordinates 5335303..5335545 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQE26_RS26000 (MQE26_26000) 5332286..5333188 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MQE26_RS26005 (MQE26_26005) 5333185..5333820 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MQE26_RS26010 (MQE26_26010) 5333817..5334746 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MQE26_RS26015 (MQE26_26015) 5334920..5335303 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MQE26_RS26020 (MQE26_26020) 5335303..5335545 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MQE26_RS26025 (MQE26_26025) 5335750..5336667 + 918 WP_004187175.1 alpha/beta hydrolase -
MQE26_RS26030 (MQE26_26030) 5336681..5337622 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MQE26_RS26035 (MQE26_26035) 5337667..5338104 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MQE26_RS26040 (MQE26_26040) 5338101..5338961 - 861 WP_004146232.1 virulence factor BrkB family protein -
MQE26_RS26045 (MQE26_26045) 5338955..5339554 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T239366 WP_002882817.1 NZ_CP094237:c5335303-5334920 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T239366 NZ_LR890693:c1304193-1303939 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT239366 WP_004150355.1 NZ_CP094237:c5335545-5335303 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT239366 NZ_LR890693:c1304441-1304190 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References