Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3321457..3322088 Replicon chromosome
Accession NZ_CP094237
Organism Klebsiella pneumoniae strain K41

Toxin (Protein)


Gene name hicA Uniprot ID A0A1Q8YTV3
Locus tag MQE26_RS16360 Protein ID WP_012542177.1
Coordinates 3321912..3322088 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2X1QFN9
Locus tag MQE26_RS16355 Protein ID WP_012542176.1
Coordinates 3321457..3321864 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQE26_RS16315 (MQE26_16315) 3318079..3318513 - 435 WP_012542168.1 phage terminase small subunit P27 family -
MQE26_RS16320 (MQE26_16320) 3318763..3319194 - 432 WP_077255824.1 hypothetical protein -
MQE26_RS16325 (MQE26_16325) 3319191..3319508 - 318 WP_020803188.1 hypothetical protein -
MQE26_RS16330 (MQE26_16330) 3319460..3319822 - 363 WP_020803185.1 HNH endonuclease -
MQE26_RS16335 (MQE26_16335) 3319806..3320012 - 207 WP_032447250.1 hypothetical protein -
MQE26_RS16340 (MQE26_16340) 3320048..3320524 - 477 WP_050516738.1 DUF2514 domain-containing protein -
MQE26_RS16345 (MQE26_16345) 3320557..3321087 - 531 WP_023328100.1 lysozyme -
MQE26_RS16350 (MQE26_16350) 3321065..3321301 - 237 WP_004111739.1 class II holin family protein -
MQE26_RS16355 (MQE26_16355) 3321457..3321864 - 408 WP_012542176.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MQE26_RS16360 (MQE26_16360) 3321912..3322088 - 177 WP_012542177.1 type II toxin-antitoxin system HicA family toxin Toxin
MQE26_RS16365 (MQE26_16365) 3322451..3323608 + 1158 WP_020803203.1 DUF262 domain-containing protein -
MQE26_RS16370 (MQE26_16370) 3323610..3324299 + 690 WP_012542179.1 MAE_28990/MAE_18760 family HEPN-like nuclease -
MQE26_RS16375 (MQE26_16375) 3324265..3325308 - 1044 WP_020803204.1 DNA cytosine methyltransferase -
MQE26_RS16380 (MQE26_16380) 3325398..3325742 - 345 WP_032447265.1 antiterminator Q family protein -
MQE26_RS16385 (MQE26_16385) 3325755..3326786 - 1032 WP_047670001.1 DUF968 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3288821..3342056 53235


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-61)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6632.83 Da        Isoelectric Point: 11.0174

>T239360 WP_012542177.1 NZ_CP094237:c3322088-3321912 [Klebsiella pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Download         Length: 177 bp

>T239360 NZ_LR890692:98168-98452 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14977.99 Da        Isoelectric Point: 4.4277

>AT239360 WP_012542176.1 NZ_CP094237:c3321864-3321457 [Klebsiella pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Download         Length: 408 bp

>AT239360 NZ_LR890692:97930-98178 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YTV3

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1QFN9

References