Detailed information of TA system TA239348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1483506..1484149
• Replicon: chromosome
• Accession: NZ_CP094237
• Organism: Klebsiella pneumoniae strain K41

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MQE26_RS07315
• Protein ID: WP_012540249.1
• Coordinates: 1483733..1484149 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: MQE26_RS07310
• Protein ID: WP_001261276.1
• Coordinates: 1483506..1483736 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE26_RS07280 (MQE26_07280)	1479251..1479940	+	690	WP_094307691.1	N-acetylmannosamine-6-phosphate 2-epimerase	-
MQE26_RS07285 (MQE26_07285)	1479937..1480812	+	876	WP_094307692.1	N-acetylmannosamine kinase	-
MQE26_RS07290 (MQE26_07290)	1480809..1481273	+	465	WP_094307693.1	N-acetylneuraminate anomerase	-
MQE26_RS07295 (MQE26_07295)	1481562..1482350	+	789	Protein_1424	sulfatase-like hydrolase/transferase	-
MQE26_RS07300 (MQE26_07300)	1482773..1483027	+	255	WP_094307695.1	hypothetical protein	-
MQE26_RS07305 (MQE26_07305)	1483033..1483374	-	342	Protein_1426	IS3 family transposase	-
MQE26_RS07310 (MQE26_07310)	1483506..1483736	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MQE26_RS07315 (MQE26_07315)	1483733..1484149	+	417	WP_012540249.1	type II toxin-antitoxin system VapC family toxin	Toxin
MQE26_RS07320 (MQE26_07320)	1484190..1485068	-	879	WP_064147992.1	restriction endonuclease	-
MQE26_RS07325 (MQE26_07325)	1485726..1487003	+	1278	WP_064147993.1	HlyD family secretion protein	-
MQE26_RS07330 (MQE26_07330)	1486993..1489101	+	2109	WP_064147994.1	peptidase domain-containing ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	iutA / iucD / iucC / iucB / iucA	1418077..1511443	93366
	-	flank	IS/Tn	-	-	1483033..1483263	230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15050.53 Da
• Isoelectric Point: 8.5403

>T239348 WP_012540249.1 NZ_CP094237:1483733-1484149 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T239348 NZ_LR890691:330576-330944 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTATTAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT239348 WP_001261276.1 NZ_CP094237:1483506-1483736 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT239348 NZ_LR890691:330358-330579 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023