Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1662..2799 Replicon plasmid pNY13320-6
Accession NZ_CP094236
Organism Enterococcus faecium strain NY13320

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag MPN36_RS15295 Protein ID WP_001284311.1
Coordinates 1936..2799 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag MPN36_RS15290 Protein ID WP_000301765.1
Coordinates 1662..1934 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPN36_RS15275 (MPN36_15275) 345..1025 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
MPN36_RS15280 (MPN36_15280) 1081..1338 + 258 Protein_2 chromosome partitioning protein ParA -
MPN36_RS15285 (MPN36_15285) 1430..1645 + 216 WP_001835296.1 peptide-binding protein -
MPN36_RS15290 (MPN36_15290) 1662..1934 + 273 WP_000301765.1 antitoxin Antitoxin
MPN36_RS15295 (MPN36_15295) 1936..2799 + 864 WP_001284311.1 zeta toxin family protein Toxin
MPN36_RS15300 (MPN36_15300) 3062..3799 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
MPN36_RS15305 (MPN36_15305) 3924..4007 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
MPN36_RS15530 4056..4139 - 84 Protein_8 MLS leader peptide -
MPN36_RS15310 (MPN36_15310) 4549..5343 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
MPN36_RS15315 (MPN36_15315) 5436..5687 - 252 Protein_10 GNAT family N-acetyltransferase -
MPN36_RS15320 (MPN36_15320) 5932..7227 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / VanHAX - 1..36298 36298
- inside IScluster/Tn erm(B) / aph(3')-III / VanHAX - 345..18556 18211


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T239343 WP_001284311.1 NZ_CP094236:1936-2799 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T239343 NZ_LR890686:c4888880-4887963 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT239343 WP_000301765.1 NZ_CP094236:1662-1934 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT239343 NZ_LR890686:c4889644-4888886 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References