Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 476700..477271 Replicon chromosome
Accession NZ_CP094230
Organism Enterococcus faecium strain NY13320

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag MPN36_RS02250 Protein ID WP_002286801.1
Coordinates 476930..477271 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag MPN36_RS02245 Protein ID WP_002323011.1
Coordinates 476700..476930 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPN36_RS02220 (MPN36_02220) 471833..473164 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
MPN36_RS02225 (MPN36_02225) 473186..473812 + 627 WP_101699687.1 cysteine hydrolase -
MPN36_RS02230 (MPN36_02230) 473995..474576 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
MPN36_RS02235 (MPN36_02235) 475308..475870 + 563 Protein_443 SOS response-associated peptidase family protein -
MPN36_RS02240 (MPN36_02240) 476075..476413 - 339 WP_002286804.1 hypothetical protein -
MPN36_RS02245 (MPN36_02245) 476700..476930 + 231 WP_002323011.1 hypothetical protein Antitoxin
MPN36_RS02250 (MPN36_02250) 476930..477271 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MPN36_RS02255 (MPN36_02255) 478121..478306 + 186 WP_002304207.1 hypothetical protein -
MPN36_RS02260 (MPN36_02260) 478811..479005 + 195 WP_002297028.1 hypothetical protein -
MPN36_RS02265 (MPN36_02265) 479196..479445 + 250 Protein_449 transposase -
MPN36_RS02270 (MPN36_02270) 479689..480519 - 831 WP_002286789.1 manganese catalase family protein -
MPN36_RS02275 (MPN36_02275) 480727..482061 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T239340 WP_002286801.1 NZ_CP094230:476930-477271 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T239340 NZ_LR890686:3991523-3991741 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT239340 WP_002323011.1 NZ_CP094230:476700-476930 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT239340 NZ_LR890686:3991122-3991496 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References