Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 227259..227781 Replicon plasmid pBio3
Accession NZ_CP094228
Organism Klebsiella pneumoniae strain Bio3

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag MOO48_RS29230 Protein ID WP_038991638.1
Coordinates 227259..227543 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag MOO48_RS29235 Protein ID WP_004181777.1
Coordinates 227533..227781 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO48_RS29195 (MOO48_29195) 222896..223240 + 345 WP_014386518.1 hypothetical protein -
MOO48_RS29200 (MOO48_29200) 223237..223608 + 372 WP_044816264.1 hypothetical protein -
MOO48_RS29205 (MOO48_29205) 223653..224840 - 1188 WP_043907031.1 RNA-guided endonuclease TnpB family protein -
MOO48_RS29210 (MOO48_29210) 224876..225304 + 429 WP_077256229.1 IS200/IS605 family transposase -
MOO48_RS29215 (MOO48_29215) 225383..225607 + 225 Protein_240 transposase -
MOO48_RS29220 (MOO48_29220) 225879..227105 - 1227 WP_040120327.1 RNA-guided endonuclease TnpB family protein -
MOO48_RS29225 (MOO48_29225) 227141..227242 + 102 Protein_242 IS200/IS605 family transposase -
MOO48_RS29230 (MOO48_29230) 227259..227543 - 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MOO48_RS29235 (MOO48_29235) 227533..227781 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MOO48_RS29240 (MOO48_29240) 228072..229871 + 1800 WP_043907032.1 ATP-dependent helicase -
MOO48_RS29245 (MOO48_29245) 230205..231191 + 987 WP_040120328.1 hypothetical protein -
MOO48_RS29250 (MOO48_29250) 231336..231689 + 354 WP_004181774.1 hypothetical protein -
MOO48_RS29255 (MOO48_29255) 231751..232572 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1A / blaOXA-232 / dfrA14 / tet(D) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 rmpA / iutA / iucD / iucC / iucB / iucA 1..328083 328083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T239338 WP_038991638.1 NZ_CP094228:c227543-227259 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T239338 NZ_LR890686:1967870-1967972 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT239338 WP_004181777.1 NZ_CP094228:c227781-227533 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT239338 NZ_LR890686:c1967979-1967834 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References