Detailed information of TA system TA239336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 57409..58079
• Replicon: plasmid pBio3
• Accession: NZ_CP094228
• Organism: Klebsiella pneumoniae strain Bio3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: MOO48_RS28350
• Protein ID: WP_004213072.1
• Coordinates: 57636..58079 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: MOO48_RS28345
• Protein ID: WP_004213073.1
• Coordinates: 57409..57639 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO48_RS28320 (MOO48_28320)	53632..54531	-	900	WP_004225022.1	nucleotide-binding protein	-
MOO48_RS28325 (MOO48_28325)	54521..54811	-	291	WP_004213078.1	hypothetical protein	-
MOO48_RS28330 (MOO48_28330)	55163..55369	+	207	WP_004213077.1	hypothetical protein	-
MOO48_RS28335 (MOO48_28335)	55359..55652	-	294	WP_004213076.1	hypothetical protein	-
MOO48_RS28340 (MOO48_28340)	55668..56801	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
MOO48_RS28345 (MOO48_28345)	57409..57639	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MOO48_RS28350 (MOO48_28350)	57636..58079	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
MOO48_RS28355 (MOO48_28355)	58228..58479	+	252	WP_227640036.1	hypothetical protein	-
MOO48_RS28360 (MOO48_28360)	58502..58806	-	305	Protein_68	transposase	-
MOO48_RS28365 (MOO48_28365)	59223..59857	+	635	Protein_69	mucoid phenotype regulator RmpA2	-
MOO48_RS28370 (MOO48_28370)	60376..60779	-	404	Protein_70	GAF domain-containing protein	-
MOO48_RS28375 (MOO48_28375)	60870..61790	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
MOO48_RS28380 (MOO48_28380)	61839..62330	-	492	WP_011251327.1	DM13 domain-containing protein	-
MOO48_RS28385 (MOO48_28385)	62393..62668	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1A / blaOXA-232 / dfrA14 / tet(D) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	rmpA / iutA / iucD / iucC / iucB / iucA	1..328083	328083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T239336 WP_004213072.1 NZ_CP094228:57636-58079 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T239336 NZ_LR890686:324412-324780 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT239336 WP_004213073.1 NZ_CP094228:57409-57639 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT239336 NZ_LR890686:324194-324415 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620