Detailed information of TA system TA239335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 11311..12062
• Replicon: plasmid pBio3
• Accession: NZ_CP094228
• Organism: Klebsiella pneumoniae strain Bio3

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: MOO48_RS28100
• Protein ID: WP_048333033.1
• Coordinates: 11580..12062 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: MOO48_RS28095
• Protein ID: WP_004902250.1
• Coordinates: 11311..11589 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO48_RS28060 (MOO48_28060)	6950..7375	-	426	WP_000522996.1	organomercurial transporter MerC	-
MOO48_RS28065 (MOO48_28065)	7403..7678	-	276	WP_043907009.1	mercury resistance system periplasmic binding protein MerP	-
MOO48_RS28070 (MOO48_28070)	7694..8059	-	366	WP_004200999.1	mercuric ion transporter MerT	-
MOO48_RS28075 (MOO48_28075)	8131..8586	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
MOO48_RS28080 (MOO48_28080)	9239..9697	+	459	WP_014386535.1	hypothetical protein	-
MOO48_RS28085 (MOO48_28085)	10528..10869	+	342	WP_032437747.1	hypothetical protein	-
MOO48_RS28090 (MOO48_28090)	10977..11189	+	213	WP_016946198.1	hypothetical protein	-
MOO48_RS28095 (MOO48_28095)	11311..11589	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
MOO48_RS28100 (MOO48_28100)	11580..12062	+	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
MOO48_RS28105 (MOO48_28105)	13097..13636	+	540	WP_004902239.1	hypothetical protein	-
MOO48_RS28110 (MOO48_28110)	13741..14133	+	393	WP_032442757.1	hypothetical protein	-
MOO48_RS28115 (MOO48_28115)	14234..14989	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
MOO48_RS28120 (MOO48_28120)	15016..15663	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1A / blaOXA-232 / dfrA14 / tet(D) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	rmpA / iutA / iucD / iucC / iucB / iucA	1..328083	328083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T239335 WP_048333033.1 NZ_CP094228:11580-12062 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T239335 NZ_LR890681:69614-69901 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT239335 WP_004902250.1 NZ_CP094228:11311-11589 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT239335 NZ_LR890681:69375-69614 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3