Detailed information of TA system TA239333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 5592057..5592867
• Replicon: chromosome
• Accession: NZ_CP094227
• Organism: Klebsiella pneumoniae strain Bio3

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A060VJ83
• Locus tag: MOO48_RS27170
• Protein ID: WP_004178461.1
• Coordinates: 5592057..5592590 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: MOO48_RS27175
• Protein ID: WP_002887278.1
• Coordinates: 5592601..5592867 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO48_RS27140 (5587562)	5587562..5588200	-	639	WP_004210261.1	VWA domain-containing protein	-
MOO48_RS27145 (5588680)	5588680..5589138	-	459	WP_004026591.1	ribbon-helix-helix protein, CopG family	-
MOO48_RS27150 (5589141)	5589141..5590112	-	972	WP_231538241.1	hypothetical protein	-
MOO48_RS27155 (5590257)	5590257..5590364	-	108	Protein_5355	DUF4236 domain-containing protein	-
MOO48_RS27160 (5590375)	5590375..5590590	-	216	Protein_5356	HpcH/HpaI aldolase/citrate lyase family protein	-
MOO48_RS27165 (5590888)	5590888..5592009	+	1122	WP_002887282.1	cupin domain-containing protein	-
MOO48_RS27170 (5592057)	5592057..5592590	-	534	WP_004178461.1	type II toxin-antitoxin system toxin KacT	Toxin
MOO48_RS27175 (5592601)	5592601..5592867	-	267	WP_002887278.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
MOO48_RS27180 (5592970)	5592970..5594403	-	1434	WP_004178459.1	Cu(+)/Ag(+) sensor histidine kinase	-
MOO48_RS27185 (5594393)	5594393..5595076	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
MOO48_RS27190 (5595248)	5595248..5596633	+	1386	WP_004178457.1	efflux transporter outer membrane subunit	-
MOO48_RS27195 (5596651)	5596651..5596995	+	345	WP_004197273.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	algU / rfaE / vgrG/tssI / clpV/tssH / hcp/tssD / dotU/tssL / vasE/tssK / vipB/tssC / vipA/tssB	5167476..5599673	432197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19810.65 Da
• Isoelectric Point: 5.2614

>T239333 WP_004178461.1 NZ_CP094227:c5592590-5592057 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T239333 NZ_LR890681:21888-22193 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT239333 WP_002887278.1 NZ_CP094227:c5592867-5592601 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT239333 NZ_LR890681:21668-21886 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A060VJ83

Antitoxin

• AlphaFold DB: A0A0H3GLZ1