Detailed information of TA system TA239332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 5577380..5578131
• Replicon: chromosome
• Accession: NZ_CP094227
• Organism: Klebsiella pneumoniae strain Bio3

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: MOO48_RS27090
• Protein ID: WP_048333033.1
• Coordinates: 5577649..5578131 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: MOO48_RS27085
• Protein ID: WP_004902250.1
• Coordinates: 5577380..5577658 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO48_RS27050 (5573019)	5573019..5573444	-	426	WP_000522996.1	organomercurial transporter MerC	-
MOO48_RS27055 (5573472)	5573472..5573747	-	276	WP_043907009.1	mercury resistance system periplasmic binding protein MerP	-
MOO48_RS27060 (5573763)	5573763..5574128	-	366	WP_004200999.1	mercuric ion transporter MerT	-
MOO48_RS27065 (5574200)	5574200..5574655	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
MOO48_RS27070 (5575308)	5575308..5575766	+	459	WP_014386535.1	hypothetical protein	-
MOO48_RS27075 (5576597)	5576597..5576938	+	342	WP_032437747.1	hypothetical protein	-
MOO48_RS27080 (5577046)	5577046..5577258	+	213	WP_016946198.1	hypothetical protein	-
MOO48_RS27085 (5577380)	5577380..5577658	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
MOO48_RS27090 (5577649)	5577649..5578131	+	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
MOO48_RS27095 (5579166)	5579166..5579705	+	540	WP_004902239.1	hypothetical protein	-
MOO48_RS27100 (5579810)	5579810..5580202	+	393	WP_032442757.1	hypothetical protein	-
MOO48_RS27105 (5580303)	5580303..5581058	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
MOO48_RS27110 (5581085)	5581085..5581732	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	algU / rfaE / vgrG/tssI / clpV/tssH / hcp/tssD / dotU/tssL / vasE/tssK / vipB/tssC / vipA/tssB	5167476..5599673	432197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T239332 WP_048333033.1 NZ_CP094227:5577649-5578131 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T239332 NZ_LR890681:c20853-20437 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT239332 WP_004902250.1 NZ_CP094227:5577380-5577658 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT239332 NZ_LR890681:c21080-20850 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3