Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4664845..4665470 Replicon chromosome
Accession NZ_CP094227
Organism Klebsiella pneumoniae strain Bio3

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MOO48_RS22410 Protein ID WP_002882817.1
Coordinates 4664845..4665228 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MOO48_RS22415 Protein ID WP_004150355.1
Coordinates 4665228..4665470 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO48_RS22395 (4662211) 4662211..4663113 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MOO48_RS22400 (4663110) 4663110..4663745 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MOO48_RS22405 (4663742) 4663742..4664671 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MOO48_RS22410 (4664845) 4664845..4665228 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MOO48_RS22415 (4665228) 4665228..4665470 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MOO48_RS22420 (4665675) 4665675..4666592 + 918 WP_004181612.1 alpha/beta hydrolase -
MOO48_RS22425 (4666606) 4666606..4667547 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MOO48_RS22430 (4667592) 4667592..4668029 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MOO48_RS22435 (4668026) 4668026..4668886 - 861 WP_004146232.1 virulence factor BrkB family protein -
MOO48_RS22440 (4668880) 4668880..4669479 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaSHV-28 iroE / rmpA / iutA / iucD / iucC / iucB / iucA 4376820..4815766 438946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T239330 WP_002882817.1 NZ_CP094227:c4665228-4664845 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T239330 NZ_LR890679:c5186564-5186181 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT239330 WP_004150355.1 NZ_CP094227:c4665470-4665228 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT239330 NZ_LR890679:c5186806-5186564 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References