Detailed information of TA system TA239326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 4182010..4182532
• Replicon: chromosome
• Accession: NZ_CP094227
• Organism: Klebsiella pneumoniae strain Bio3

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A0C7KG00
• Locus tag: MOO48_RS19980
• Protein ID: WP_038991638.1
• Coordinates: 4182248..4182532 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: MOO48_RS19975
• Protein ID: WP_004181777.1
• Coordinates: 4182010..4182258 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO48_RS19955 (4177219)	4177219..4178040	-	822	WP_004181772.1	hypothetical protein	-
MOO48_RS19960 (4178102)	4178102..4178455	-	354	WP_004181774.1	hypothetical protein	-
MOO48_RS19965 (4178600)	4178600..4179586	-	987	WP_040120328.1	hypothetical protein	-
MOO48_RS19970 (4179920)	4179920..4181719	-	1800	WP_043907032.1	ATP-dependent helicase	-
MOO48_RS19975 (4182010)	4182010..4182258	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MOO48_RS19980 (4182248)	4182248..4182532	+	285	WP_038991638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MOO48_RS19985 (4182549)	4182549..4182650	-	102	Protein_3928	IS200/IS605 family transposase	-
MOO48_RS19990 (4182686)	4182686..4183912	+	1227	WP_040120327.1	RNA-guided endonuclease TnpB family protein	-
MOO48_RS19995 (4184184)	4184184..4184408	-	225	Protein_3930	transposase	-
MOO48_RS20000 (4184487)	4184487..4184915	-	429	WP_077256229.1	IS200/IS605 family transposase	-
MOO48_RS20005 (4184951)	4184951..4186138	+	1188	WP_043907031.1	RNA-guided endonuclease TnpB family protein	-
MOO48_RS20010 (4186183)	4186183..4186554	-	372	WP_044816264.1	hypothetical protein	-
MOO48_RS20015 (4186551)	4186551..4186895	-	345	WP_014386518.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4153674..4186138	32464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10993.73 Da
• Isoelectric Point: 10.6516

>T239326 WP_038991638.1 NZ_CP094227:4182248-4182532 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Nucleotide

• Length: 285 bp

>T239326 NZ_LR890679:4034302-4034520 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT239326 WP_004181777.1 NZ_CP094227:4182010-4182258 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT239326 NZ_LR890679:4033901-4034275 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0C7KG00

Antitoxin

• AlphaFold DB: A0A2J4R0U8