Detailed information of TA system TA239305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapD-relB/-
• Location: 576867..577524
• Replicon: chromosome
• Accession: NZ_CP094171
• Organism: Helicobacter pylori strain Hpfe0003

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: T5D7N9
• Locus tag: MPG20_RS02805
• Protein ID: WP_000271050.1
• Coordinates: 577240..577524 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MPG20_RS02800
• Protein ID: WP_245074750.1
• Coordinates: 576867..577259 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPG20_RS02770 (MPG20_02770)	571953..572183	+	231	WP_129074199.1	HypC/HybG/HupF family hydrogenase formation chaperone	-
MPG20_RS02775 (MPG20_02775)	572202..573314	+	1113	WP_245074748.1	hydrogenase formation protein HypD	-
MPG20_RS02780 (MPG20_02780)	573265..573855	-	591	Protein_528	helicase DnaB modulator	-
MPG20_RS02795 (MPG20_02795)	574302..576503	+	2202	WP_245074749.1	Hop family adhesin BabA	-
MPG20_RS02800 (MPG20_02800)	576867..577259	+	393	WP_245074750.1	hypothetical protein	Antitoxin
MPG20_RS02805 (MPG20_02805)	577240..577524	+	285	WP_000271050.1	endoribonuclease VapD	Toxin
MPG20_RS02810 (MPG20_02810)	577699..578223	-	525	WP_121297461.1	acyl-CoA thioesterase	-
MPG20_RS02815 (MPG20_02815)	578364..579209	+	846	WP_245074751.1	SDR family oxidoreductase	-
MPG20_RS02820 (MPG20_02820)	579202..580182	+	981	WP_000921452.1	iron ABC transporter permease	-
MPG20_RS02825 (MPG20_02825)	580182..580949	+	768	WP_000242365.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11188.74 Da
• Isoelectric Point: 4.8896

>T239305 WP_000271050.1 NZ_CP094171:577240-577524 [Helicobacter pylori]
MYALAFDLKIEILKKEYGEPYNKAYDDLRQELELLGFEWTQGSVYVNYSKENTLAQVYKAINKLSQIEWFKKSVRDIRAF
KVEDFSDFTEIVKS

Nucleotide

• Length: 285 bp

>T239305 NZ_LR890669:783652-784062 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15266.25 Da
• Isoelectric Point: 7.7126

>AT239305 WP_245074750.1 NZ_CP094171:576867-577259 [Helicobacter pylori]
MPNSPTKKDYTRYSEKQLFNLINQLERKIKKMQNDRASFKEKMAKELEKRDQNFKDKIDALNELLQKISQAFDNKRDCCF
GHETPNLETQQAMREALNGVNLTQIDSLDDLTNELKRENSKGFENVCFSV

Nucleotide

• Length: 393 bp

>AT239305 NZ_LR890669:783405-783671 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 3UI3