Detailed information of TA system TA023924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4000385..4001169
• Replicon: chromosome
• Accession: NC_012880
• Organism: Dickeya dadantii Ech703

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: C6C392
• Locus tag: DD703_RS17060
• Protein ID: WP_015855088.1
• Coordinates: 4000385..4000879 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: C6C393
• Locus tag: DD703_RS17065
• Protein ID: WP_015855089.1
• Coordinates: 4000876..4001169 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DD703_RS17035	3995729..3995971	-	243	WP_023638472.1	helix-turn-helix domain-containing protein	-
DD703_RS20750	3996092..3996352	+	261	WP_012883609.1	helix-turn-helix domain-containing protein	-
DD703_RS20755	3996456..3996716	+	261	WP_015855084.1	helix-turn-helix domain-containing protein	-
DD703_RS17045	3996772..3997359	+	588	WP_015855085.1	hypothetical protein	-
DD703_RS17050	3997463..3998761	+	1299	WP_015855086.1	DUF3644 domain-containing protein	-
DD703_RS17055	3999004..4000146	-	1143	WP_015855087.1	Fic family protein	-
DD703_RS17060	4000385..4000879	-	495	WP_015855088.1	GNAT family N-acetyltransferase	Toxin
DD703_RS17065	4000876..4001169	-	294	WP_015855089.1	DUF1778 domain-containing protein	Antitoxin
DD703_RS17070	4001447..4002166	+	720	WP_015855090.1	hypothetical protein	-
DD703_RS17075	4002234..4002812	+	579	WP_071597711.1	RES family NAD+ phosphorylase	-
DD703_RS20760	4003195..4003550	+	356	Protein_3380	integrase arm-type DNA-binding domain-containing protein	-
DD703_RS17080	4003683..4003967	+	285	WP_015855092.1	DUF134 domain-containing protein	-
DD703_RS17085	4004079..4004576	+	498	WP_015855093.1	hypothetical protein	-
DD703_RS17090	4004647..4005243	-	597	WP_015855094.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (84-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17584.43 Da
• Isoelectric Point: 7.8046

>T23924 WP_015855088.1 NC_012880:c4000879-4000385 [Dickeya paradisiaca Ech703]
MISAPEPLHAEHVLSSFCCGVESMDNWLKLRAMKNQVTGASRTFVSCDGSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYIRVGFEPSPIDPMMLMVTLGDLVG
SLSS

Nucleotide

• Length: 495 bp

>T23924 NC_012880:c4000879-4000385 [Dickeya paradisiaca Ech703]
ATGATCTCCGCCCCTGAGCCACTCCACGCCGAACATGTACTTTCCTCATTCTGTTGCGGCGTGGAGTCTATGGATAACTG
GCTAAAACTACGGGCGATGAAAAATCAGGTCACTGGCGCATCGCGAACTTTCGTCAGTTGCGATGGTTCAAAAGTGCTGG
CGTATTATTCATTGGCTTCCAGCGCCGTAGCAACGAATGCAGCACCCGGACGTTTTCGCCGCAATATGCCCGATCCGATC
CCGGTGGTAGTGCTGGGACGTCTGGCAGTAGATAAATCATTGCATGGGCAGGGCGTTGGCCGAGCGCTGGTACGTGATGC
CGGGCTACGGGTGATTCAGGTGGCGGATACTATCGGCATTCGCGGGATGTTGGTTCATGCGCTATCTGATGAAGCGCGGG
AGTTTTATATACGTGTGGGGTTTGAGCCTTCGCCGATAGATCCGATGATGCTGATGGTGACGTTGGGGGATTTGGTGGGG
AGTTTATCAAGTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10889.43 Da
• Isoelectric Point: 10.1968

>AT23924 WP_015855089.1 NC_012880:c4001169-4000876 [Dickeya paradisiaca Ech703]
MSATNNVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT23924 NC_012880:c4001169-4000876 [Dickeya paradisiaca Ech703]
ATGTCAGCGACGAACAACGTTTCTGTGAAACGCGAAACATTGAATTTACGCATCAAACCCGCCGAACGCGATCTGATTGA
TCGTGCAGCGAAGGCCAGAGGGAAAAATCGCACTGATTTTGTTCTTGAGGCTGCTCGCGCTGCCGCAGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCGGATCCTGACGCTTATCAGGAATTCCTTGCGCGCTTGGATCAGGCTCCTTCGCCGAAT
GCCGCGCTGCGCAAAACCATGCAGACGCCTGCACCGTGGGAACAGAAAAAATGA

Structures

Toxin

• AlphaFold DB: C6C392

Antitoxin

• AlphaFold DB: C6C393