Detailed information of TA system TA239141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 315276..315810
• Replicon: chromosome
• Accession: NZ_CP093957
• Organism: Enterococcus faecalis strain NY13321

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: MPM64_RS01435
• Protein ID: WP_002360769.1
• Coordinates: 315276..315551 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: MPM64_RS01440
• Protein ID: WP_002369771.1
• Coordinates: 315544..315810 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPM64_RS01410 (310890)	310890..311864	+	975	WP_002355428.1	choloylglycine hydrolase	-
MPM64_RS01415 (312533)	312533..313141	-	609	Protein_284	IS6 family transposase	-
MPM64_RS01420 (313173)	313173..313358	-	186	WP_002358660.1	hypothetical protein	-
MPM64_RS01425 (313426)	313426..314320	-	895	Protein_286	IS256 family transposase	-
MPM64_RS01430 (314441)	314441..315085	+	645	WP_002377952.1	IS6 family transposase	-
MPM64_RS01435 (315276)	315276..315551	-	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
MPM64_RS01440 (315544)	315544..315810	-	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MPM64_RS01445 (315921)	315921..316505	-	585	WP_226013793.1	thermonuclease family protein	-
MPM64_RS01450 (316529)	316529..316945	-	417	WP_010710134.1	single-stranded DNA-binding protein	-
MPM64_RS01455 (317021)	317021..317146	-	126	WP_229023520.1	DUF3850 domain-containing protein	-
MPM64_RS01460 (317283)	317283..317783	-	501	WP_002360775.1	DnaJ domain-containing protein	-
MPM64_RS01465 (317816)	317816..318082	-	267	WP_002377947.1	hypothetical protein	-
MPM64_RS01470 (318674)	318674..319162	-	489	WP_010710133.1	hypothetical protein	-
MPM64_RS01475 (319168)	319168..319761	-	594	WP_002414802.1	replication-relaxation family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(6')-aph(2'')	cylB / cylM / cylS / cylL / cylR1 / cylR2 / bsh	276158..329779	53621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T239141 WP_002360769.1 NZ_CP093957:c315551-315276 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T239141 NZ_LR890616:329003-329371 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT239141 WP_002369771.1 NZ_CP093957:c315810-315544 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT239141 NZ_LR890616:328785-329006 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7