Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 31026..32163 Replicon plasmid pNY11066-2
Accession NZ_CP093949
Organism Enterococcus faecium strain NY11066

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag MPL63_RS15635 Protein ID WP_001284311.1
Coordinates 31026..31889 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag MPL63_RS15640 Protein ID WP_000301765.1
Coordinates 31891..32163 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPL63_RS15610 (MPL63_15610) 26598..27893 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
MPL63_RS15615 (MPL63_15615) 28138..28389 + 252 Protein_32 GNAT family N-acetyltransferase -
MPL63_RS15620 (MPL63_15620) 28482..29276 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
MPL63_RS15845 29686..29769 + 84 Protein_34 MLS leader peptide -
MPL63_RS15625 (MPL63_15625) 29818..29901 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
MPL63_RS15630 (MPL63_15630) 30026..30763 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
MPL63_RS15635 (MPL63_15635) 31026..31889 - 864 WP_001284311.1 zeta toxin family protein Toxin
MPL63_RS15640 (MPL63_15640) 31891..32163 - 273 WP_000301765.1 antitoxin Antitoxin
MPL63_RS15645 (MPL63_15645) 32180..32395 - 216 WP_001835296.1 peptide-binding protein -
MPL63_RS15650 (MPL63_15650) 32487..32744 - 258 Protein_40 chromosome partitioning protein ParA -
MPL63_RS15655 (MPL63_15655) 32800..33480 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
MPL63_RS15660 (MPL63_15660) 34012..35112 + 1101 WP_224198038.1 cysteine protease staphopain -
MPL63_RS15665 (MPL63_15665) 35085..35528 + 444 WP_002318191.1 hypothetical protein -
MPL63_RS15670 (MPL63_15670) 35830..36210 + 381 WP_002348702.1 MobC family plasmid mobilization relaxosome protein -
MPL63_RS15675 (MPL63_15675) 36192..37106 + 915 WP_002348701.1 relaxase/mobilization nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / aph(3')-III / erm(B) - 1..42035 42035
- inside IScluster/Tn VanHAX / aph(3')-III / erm(B) - 15559..33480 17921


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T239132 WP_001284311.1 NZ_CP093949:c31889-31026 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T239132 NZ_LR890606:c4276997-4276647 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT239132 WP_000301765.1 NZ_CP093949:c32163-31891 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT239132 NZ_LR890606:c4277242-4276991 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References