Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | Txe-Axe/Txe-RelB |
| Location | 178837..179356 | Replicon | plasmid pNY11066-1 |
| Accession | NZ_CP093948 | ||
| Organism | Enterococcus faecium strain NY11066 | ||
Toxin (Protein)
| Gene name | Txe | Uniprot ID | Q848U6 |
| Locus tag | MPL63_RS15270 | Protein ID | WP_000588503.1 |
| Coordinates | 178837..179094 (-) | Length | 86 a.a. |
Antitoxin (Protein)
| Gene name | Axe | Uniprot ID | Q83WJ8 |
| Locus tag | MPL63_RS15275 | Protein ID | WP_000388479.1 |
| Coordinates | 179087..179356 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MPL63_RS15230 (MPL63_15230) | 173895..174131 | - | 237 | WP_224198013.1 | bacteriophage abortive infection AbiH family protein | - |
| MPL63_RS15235 (MPL63_15235) | 174155..174769 | - | 615 | WP_065305703.1 | recombinase family protein | - |
| MPL63_RS15240 (MPL63_15240) | 174922..175512 | - | 591 | WP_065305702.1 | recombinase family protein | - |
| MPL63_RS15245 (MPL63_15245) | 175723..175926 | - | 204 | WP_002299573.1 | hypothetical protein | - |
| MPL63_RS15250 (MPL63_15250) | 175936..176358 | - | 423 | WP_002303116.1 | hypothetical protein | - |
| MPL63_RS15255 (MPL63_15255) | 176596..177210 | + | 615 | WP_001261742.1 | recombinase family protein | - |
| MPL63_RS15260 (MPL63_15260) | 177375..178199 | - | 825 | WP_001809248.1 | hypothetical protein | - |
| MPL63_RS15265 (MPL63_15265) | 178223..178378 | - | 156 | WP_002347142.1 | hypothetical protein | - |
| MPL63_RS15270 (MPL63_15270) | 178837..179094 | - | 258 | WP_000588503.1 | Txe/YoeB family addiction module toxin | Toxin |
| MPL63_RS15275 (MPL63_15275) | 179087..179356 | - | 270 | WP_000388479.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| MPL63_RS15280 (MPL63_15280) | 179487..179666 | - | 180 | WP_001120991.1 | hypothetical protein | - |
| MPL63_RS15285 (MPL63_15285) | 179682..179882 | - | 201 | WP_002347143.1 | hypothetical protein | - |
| MPL63_RS15290 (MPL63_15290) | 180047..180754 | - | 708 | WP_002303117.1 | hypothetical protein | - |
| MPL63_RS15295 (MPL63_15295) | 180747..181184 | - | 438 | WP_002300851.1 | hypothetical protein | - |
| MPL63_RS15300 (MPL63_15300) | 181199..181450 | - | 252 | WP_002296238.1 | hypothetical protein | - |
| MPL63_RS15305 (MPL63_15305) | 181450..181656 | - | 207 | WP_002347218.1 | hypothetical protein | - |
| MPL63_RS15310 (MPL63_15310) | 182093..182353 | - | 261 | WP_002295623.1 | hypothetical protein | - |
| MPL63_RS15315 (MPL63_15315) | 182527..182631 | + | 105 | WP_008274030.1 | putative holin-like toxin | - |
| MPL63_RS15320 (MPL63_15320) | 182772..183257 | - | 486 | WP_002303313.1 | single-stranded DNA-binding protein | - |
| MPL63_RS15325 (MPL63_15325) | 183260..184159 | - | 900 | WP_002303312.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(6')-aph(2'') | - | 1..210381 | 210381 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 86 a.a. Molecular weight: 10206.53 Da Isoelectric Point: 8.4369
>T239131 WP_000588503.1 NZ_CP093948:c179094-178837 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY
Download Length: 258 bp
>T239131 NZ_LR890606:c4265586-4265296 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10373.51 Da Isoelectric Point: 4.4346
>AT239131 WP_000388479.1 NZ_CP093948:c179356-179087 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD
Download Length: 270 bp
>AT239131 NZ_LR890606:c4265818-4265576 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGACTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGACTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A828ZXQ8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A828ZWU7 |