Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 487467..488038 Replicon chromosome
Accession NZ_CP093947
Organism Enterococcus faecium strain NY11066

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag MPL63_RS02285 Protein ID WP_002286801.1
Coordinates 487697..488038 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag MPL63_RS02280 Protein ID WP_002323011.1
Coordinates 487467..487697 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPL63_RS02255 (MPL63_02255) 482600..483931 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
MPL63_RS02260 (MPL63_02260) 483953..484579 + 627 WP_002286816.1 cysteine hydrolase -
MPL63_RS02265 (MPL63_02265) 484762..485343 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
MPL63_RS02270 (MPL63_02270) 486075..486637 + 563 Protein_450 SOS response-associated peptidase family protein -
MPL63_RS02275 (MPL63_02275) 486842..487180 - 339 WP_002286804.1 hypothetical protein -
MPL63_RS02280 (MPL63_02280) 487467..487697 + 231 WP_002323011.1 hypothetical protein Antitoxin
MPL63_RS02285 (MPL63_02285) 487697..488038 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MPL63_RS02290 (MPL63_02290) 488888..489073 + 186 WP_002304207.1 hypothetical protein -
MPL63_RS02295 (MPL63_02295) 489578..489772 + 195 WP_002297028.1 hypothetical protein -
MPL63_RS02300 (MPL63_02300) 489963..490212 + 250 Protein_456 transposase -
MPL63_RS02305 (MPL63_02305) 490456..491286 - 831 WP_002286789.1 manganese catalase family protein -
MPL63_RS02310 (MPL63_02310) 491494..492828 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T239129 WP_002286801.1 NZ_CP093947:487697-488038 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T239129 NZ_LR890606:c4119481-4119194 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT239129 WP_002323011.1 NZ_CP093947:487467-487697 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT239129 NZ_LR890606:c4119207-4118905 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References