Detailed information of TA system TA239127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 24728..25836
• Replicon: plasmid pNY2032-2
• Accession: NZ_CP093943
• Organism: Enterococcus faecium strain NY2032

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: MPL11_RS15205
• Protein ID: WP_000233000.1
• Coordinates: 24967..25836 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: MPL11_RS15200
• Protein ID: WP_000205227.1
• Coordinates: 24728..24952 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPL11_RS15175 (MPL11_15175)	20187..21038	+	852	WP_278189526.1	ATP-binding cassette domain-containing protein	-
MPL11_RS15180 (MPL11_15180)	21068..21748	-	681	WP_001067799.1	IS6-like element IS1216 family transposase	-
MPL11_RS15185 (MPL11_15185)	22107..22604	+	498	WP_002327635.1	DNA recombinase	-
MPL11_RS15190 (MPL11_15190)	22605..23021	+	417	WP_000323438.1	recombinase	-
MPL11_RS15195 (MPL11_15195)	23023..24585	+	1563	WP_000136908.1	recombinase family protein	-
MPL11_RS15200 (MPL11_15200)	24728..24952	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
MPL11_RS15205 (MPL11_15205)	24967..25836	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
MPL11_RS15210 (MPL11_15210)	25817..26551	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
MPL11_RS15215 (MPL11_15215)	26584..27447	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
MPL11_RS15220 (MPL11_15220)	27491..28018	+	528	WP_002294507.1	phosphoribosyltransferase family protein	-
MPL11_RS15225 (MPL11_15225)	28246..29565	+	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
MPL11_RS15230 (MPL11_15230)	29717..30724	+	1008	Protein_32	DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T239127 WP_000233000.1 NZ_CP093943:24967-25836 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T239127 NZ_LR890606:3551135-3551353 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT239127 WP_000205227.1 NZ_CP093943:24728-24952 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT239127 NZ_LR890606:3550735-3551109 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: R3JIN1