Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB-HicA
Location 169446..170063 Replicon plasmid pNY2032-1
Accession NZ_CP093942
Organism Enterococcus faecium strain NY2032

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag MPL11_RS14595 Protein ID WP_224451579.1
Coordinates 169911..170063 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A829EWE8
Locus tag MPL11_RS14590 Protein ID WP_002305052.1
Coordinates 169446..169877 (-) Length 144 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPL11_RS14575 (MPL11_14575) 165261..166688 - 1428 WP_002322784.1 glycoside hydrolase family 32 protein -
MPL11_RS14580 (MPL11_14580) 166742..168016 - 1275 WP_002323715.1 MFS transporter -
MPL11_RS14585 (MPL11_14585) 168319..169272 + 954 WP_242815714.1 IS30 family transposase -
MPL11_RS14590 (MPL11_14590) 169446..169877 - 432 WP_002305052.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MPL11_RS14595 (MPL11_14595) 169911..170063 - 153 WP_224451579.1 type II toxin-antitoxin system HicA family toxin Toxin
MPL11_RS14600 (MPL11_14600) 170157..170336 - 180 Protein_183 hypothetical protein -
MPL11_RS14605 (MPL11_14605) 170352..170552 - 201 WP_002324500.1 hypothetical protein -
MPL11_RS14610 (MPL11_14610) 170717..171412 - 696 WP_002305050.1 hypothetical protein -
MPL11_RS14615 (MPL11_14615) 171405..171842 - 438 WP_002305049.1 hypothetical protein -
MPL11_RS14620 (MPL11_14620) 171858..172109 - 252 WP_002287517.1 hypothetical protein -
MPL11_RS14625 (MPL11_14625) 172109..172315 - 207 WP_002324499.1 hypothetical protein -
MPL11_RS14630 (MPL11_14630) 172759..173019 - 261 WP_002326514.1 hypothetical protein -
MPL11_RS14635 (MPL11_14635) 173152..173296 + 145 Protein_190 type I toxin-antitoxin system toxin PepG1 -
MPL11_RS14640 (MPL11_14640) 173455..173658 - 204 Protein_191 hypothetical protein -
MPL11_RS14645 (MPL11_14645) 173653..173739 - 87 Protein_192 IS200/IS605 family transposase -
MPL11_RS14650 (MPL11_14650) 174007..174483 - 477 WP_002305045.1 PTS glucose transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..255599 255599
- flank IS/Tn - - 168319..169272 953


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(2-49)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(10-104)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5815.65 Da        Isoelectric Point: 10.3560

>T239126 WP_224451579.1 NZ_CP093942:c170063-169911 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Download         Length: 153 bp

>T239126 NZ_LR890606:2757071-2757178 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 144 a.a.        Molecular weight: 15811.92 Da        Isoelectric Point: 4.1638

>AT239126 WP_002305052.1 NZ_CP093942:c169877-169446 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Download         Length: 432 bp

>AT239126 NZ_LR890606:c2757024-2756958 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829EWE8

References