Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 534839..535410 Replicon chromosome
Accession NZ_CP093941
Organism Enterococcus faecium strain NY2032

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag MPL11_RS02545 Protein ID WP_002286801.1
Coordinates 535069..535410 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag MPL11_RS02540 Protein ID WP_002323011.1
Coordinates 534839..535069 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPL11_RS02515 (MPL11_02515) 530095..531426 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
MPL11_RS02520 (MPL11_02520) 531448..532074 + 627 WP_101699687.1 cysteine hydrolase -
MPL11_RS02525 (MPL11_02525) 532257..532838 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
MPL11_RS02530 (MPL11_02530) 533447..534009 + 563 Protein_502 SOS response-associated peptidase family protein -
MPL11_RS02535 (MPL11_02535) 534214..534552 - 339 WP_002286804.1 hypothetical protein -
MPL11_RS02540 (MPL11_02540) 534839..535069 + 231 WP_002323011.1 hypothetical protein Antitoxin
MPL11_RS02545 (MPL11_02545) 535069..535410 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MPL11_RS02550 (MPL11_02550) 536259..536444 + 186 WP_002304207.1 hypothetical protein -
MPL11_RS02555 (MPL11_02555) 536949..537143 + 195 WP_002297028.1 hypothetical protein -
MPL11_RS02560 (MPL11_02560) 537334..537583 + 250 Protein_508 transposase -
MPL11_RS02565 (MPL11_02565) 537827..538657 - 831 WP_002286789.1 manganese catalase family protein -
MPL11_RS02570 (MPL11_02570) 538865..540199 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T239125 WP_002286801.1 NZ_CP093941:535069-535410 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T239125 NZ_LR890606:2756536-2756643 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT239125 WP_002323011.1 NZ_CP093941:534839-535069 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT239125 NZ_LR890606:c2756489-2756423 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References