Detailed information of TA system TA239114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2163926..2164455
• Replicon: chromosome
• Accession: NZ_CP093935
• Organism: Staphylococcus aureus strain WA121-2021_16354

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MQP46_RS10875
• Protein ID: WP_000621175.1
• Coordinates: 2163926..2164288 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MQP46_RS10880
• Protein ID: WP_000948331.1
• Coordinates: 2164285..2164455 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQP46_RS10855 (2160904)	2160904..2161674	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MQP46_RS10860 (2161649)	2161649..2162128	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MQP46_RS10865 (2162130)	2162130..2162456	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MQP46_RS10870 (2162575)	2162575..2163576	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MQP46_RS10875 (2163926)	2163926..2164288	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MQP46_RS10880 (2164285)	2164285..2164455	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MQP46_RS10885 (2164540)	2164540..2165688	-	1149	WP_001281154.1	alanine racemase	-
MQP46_RS10890 (2165754)	2165754..2166113	-	360	WP_000581197.1	holo-ACP synthase	-
MQP46_RS10895 (2166117)	2166117..2166608	-	492	WP_001205912.1	PH domain-containing protein	-
MQP46_RS10900 (2166595)	2166595..2168178	-	1584	WP_001294626.1	PH domain-containing protein	-
MQP46_RS10905 (2168171)	2168171..2168650	-	480	WP_001287087.1	hypothetical protein	-
MQP46_RS10910 (2168858)	2168858..2169418	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T239114 WP_000621175.1 NZ_CP093935:c2164288-2163926 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T239114 NZ_LR890606:c673856-673392 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT239114 WP_000948331.1 NZ_CP093935:c2164455-2164285 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT239114 NZ_LR890606:c674191-673856 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1