Detailed information of TA system TA023911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1733169..1733803
• Replicon: chromosome
• Accession: NC_012880
• Organism: Dickeya dadantii Ech703

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DD703_RS07305
• Protein ID: WP_012765079.1
• Coordinates: 1733169..1733573 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: DD703_RS07310
• Protein ID: WP_012765080.1
• Coordinates: 1733573..1733803 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DD703_RS07290	1729077..1730951	+	1875	WP_012765076.1	FAD/NAD(P)-binding protein	-
DD703_RS07295	1730956..1731570	+	615	WP_012765077.1	cysteine hydrolase	-
DD703_RS07300	1731567..1732838	+	1272	WP_012765078.1	MFS transporter	-
DD703_RS07305	1733169..1733573	-	405	WP_012765079.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DD703_RS07310	1733573..1733803	-	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DD703_RS20425	1733897..1734176	-	280	Protein_1444	DUF4942 domain-containing protein	-
DD703_RS07320	1734637..1735500	-	864	WP_012765082.1	D-amino-acid transaminase	-
DD703_RS07325	1735871..1737106	+	1236	WP_012765083.1	alanine transaminase	-
DD703_RS07330	1737458..1737865	+	408	WP_012765084.1	GspS family T2SS pilot lipoprotein variant OutS	-
DD703_RS07335	1737910..1738491	-	582	WP_023638232.1	type II secretion system assembly factor GspB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15077.34 Da
• Isoelectric Point: 8.0239

>T23911 WP_012765079.1 NC_012880:c1733573-1733169 [Dickeya paradisiaca Ech703]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISAITLMELIYGAEKSVNPEKNLAVIEGFSARLEVQPYGFDAAVHT
GQIRAELAKQGTSIGPYDAMLAGHARFAGLILVTNNVREFERVPGLRVENWVSR

Nucleotide

• Length: 405 bp

>T23911 NC_012880:c1733573-1733169 [Dickeya paradisiaca Ech703]
ATGCTGAAATATCTGCTCGATACCAATATCTGTATTTACACCATCAAGAACAAACCGCAGGAAGTCAGGGACGCTTTCTA
CCGTCATTACGGGCAGTTTGCTATCAGTGCTATCACGCTGATGGAACTGATATATGGCGCGGAGAAATCGGTAAACCCAG
AAAAAAATCTGGCAGTGATTGAAGGATTTTCTGCACGTTTGGAAGTGCAACCTTATGGTTTTGACGCTGCCGTACATACC
GGCCAAATTCGTGCGGAACTGGCAAAACAAGGAACCTCTATCGGCCCGTATGATGCCATGTTAGCCGGTCACGCGCGGTT
CGCAGGTTTAATACTGGTAACGAACAATGTACGCGAATTTGAGCGTGTACCTGGGCTACGAGTGGAAAACTGGGTCAGTC
GATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT23911 WP_012765080.1 NC_012880:c1733803-1733573 [Dickeya paradisiaca Ech703]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT23911 NC_012880:c1733803-1733573 [Dickeya paradisiaca Ech703]
ATGGAAAAAACCACAGTCTTTAAAAGCAATCGTAGCCAGGCGGTACGACTCCCTAAAGCTGTTGCACTGCCCGATGATGT
GAAGCAGGTTGATATCGTCGCGATTGGTCGCACACGTATCATCACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTCGAAG
AGGATGGCGTAACACCTGACTTTATGATCACCAGAGAGCAACCCGATGATCAGATCAGGGAAGATTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4