Detailed information of TA system TA239095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2159598..2160127
• Replicon: chromosome
• Accession: NZ_CP093933
• Organism: Staphylococcus aureus strain WA121-2021_15363

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MQP45_RS10865
• Protein ID: WP_000621175.1
• Coordinates: 2159598..2159960 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MQP45_RS10870
• Protein ID: WP_000948331.1
• Coordinates: 2159957..2160127 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQP45_RS10845 (2156576)	2156576..2157346	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MQP45_RS10850 (2157321)	2157321..2157800	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MQP45_RS10855 (2157802)	2157802..2158128	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MQP45_RS10860 (2158247)	2158247..2159248	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MQP45_RS10865 (2159598)	2159598..2159960	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MQP45_RS10870 (2159957)	2159957..2160127	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MQP45_RS10875 (2160212)	2160212..2161360	-	1149	WP_001281154.1	alanine racemase	-
MQP45_RS10880 (2161426)	2161426..2161785	-	360	WP_000581197.1	holo-ACP synthase	-
MQP45_RS10885 (2161789)	2161789..2162280	-	492	WP_001205912.1	PH domain-containing protein	-
MQP45_RS10890 (2162267)	2162267..2163850	-	1584	WP_001294626.1	PH domain-containing protein	-
MQP45_RS10895 (2163843)	2163843..2164322	-	480	WP_001287087.1	hypothetical protein	-
MQP45_RS10900 (2164530)	2164530..2165090	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T239095 WP_000621175.1 NZ_CP093933:c2159960-2159598 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T239095 NZ_LR890603:2067185-2067288 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT239095 WP_000948331.1 NZ_CP093933:c2160127-2159957 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT239095 NZ_LR890603:c2067326-2067055 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1