Detailed information of TA system TA023908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 417053..417609
• Replicon: plasmid pR132502
• Accession: NC_012858
• Organism: Rhizobium leguminosarum bv. trifolii WSM1325

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: C6B7E2
• Locus tag: RLEG_RS28775
• Protein ID: WP_003554321.1
• Coordinates: 417053..417298 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C6B7E3
• Locus tag: RLEG_RS28780
• Protein ID: WP_003554323.1
• Coordinates: 417295..417609 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG_RS28740	412785..413048	-	264	WP_041935829.1	hypothetical protein	-
RLEG_RS28745	413407..413655	+	249	WP_003554311.1	hypothetical protein	-
RLEG_RS28750	413854..414093	+	240	WP_003554314.1	hypothetical protein	-
RLEG_RS36465	414251..414427	-	177	WP_080518593.1	hypothetical protein	-
RLEG_RS28755	414481..414807	+	327	WP_003554317.1	hypothetical protein	-
RLEG_RS38240	414981..415358	+	378	WP_041935802.1	hypothetical protein	-
RLEG_RS28765	415672..416368	+	697	Protein_394	SOS response-associated peptidase	-
RLEG_RS28770	416388..416597	+	210	WP_003554319.1	hypothetical protein	-
RLEG_RS28775	417053..417298	+	246	WP_003554321.1	BrnT family toxin	Toxin
RLEG_RS28780	417295..417609	+	315	WP_003554323.1	BrnA antitoxin family protein	Antitoxin
RLEG_RS28785	418407..418793	-	387	WP_003554325.1	DUF983 domain-containing protein	-
RLEG_RS37665	419022..419204	-	183	WP_148226366.1	hypothetical protein	-
RLEG_RS28790	419315..420289	+	975	WP_003554327.1	GTP-binding protein	-
RLEG_RS28795	420323..420820	+	498	WP_003554329.1	DUF1097 domain-containing protein	-
RLEG_RS28800	420850..421590	+	741	WP_003554331.1	nitrile hydratase subunit beta	-
RLEG_RS28805	421618..422235	+	618	WP_003554333.1	nitrile hydratase subunit alpha	-
RLEG_RS28810	422246..422572	+	327	WP_012763449.1	nitrile hydratase accessory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..660973	660973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8955.54 Da
• Isoelectric Point: 10.2690

>T23908 WP_003554321.1 NC_012858:417053-417298 [Rhizobium leguminosarum bv. trifolii WSM1325]
MKITYDEAKRRTNIEKHGLDFADLDLLFFAGSVVVPAKGERLMAIGVFRDGVIAVVFVTLGSEAISVISMRKASRKERSI
L

Nucleotide

• Length: 246 bp

>T23908 NC_012858:417053-417298 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGAAGATCACCTACGACGAAGCAAAGCGCCGGACCAATATCGAAAAGCACGGGCTCGACTTTGCCGATCTCGATCTGCT
TTTCTTTGCAGGCTCCGTTGTCGTCCCGGCCAAAGGCGAACGCCTCATGGCCATCGGTGTCTTTCGTGATGGCGTGATTG
CCGTCGTGTTCGTCACCCTCGGCTCTGAAGCCATTTCGGTAATCTCCATGCGCAAAGCCAGCCGTAAGGAAAGGAGCATC
CTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11306.85 Da
• Isoelectric Point: 6.3985

>AT23908 WP_003554323.1 NC_012858:417295-417609 [Rhizobium leguminosarum bv. trifolii WSM1325]
MTRIADSVLKISDAEEAKIQRQIASDPDAPEATDEQLAKAKPFAEALPELAESIRKNLGGRPKSNNPKVAVSIRLDPEVV
DAFKAKGEGWQSRINETLRKAVGL

Nucleotide

• Length: 315 bp

>AT23908 NC_012858:417295-417609 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGACCCGCATCGCAGATAGTGTTCTGAAAATCTCCGACGCTGAAGAAGCGAAGATCCAGAGGCAAATCGCCAGCGATCC
CGACGCCCCAGAGGCGACGGATGAACAGTTGGCCAAGGCCAAACCTTTTGCCGAAGCCCTTCCCGAGTTGGCCGAGTCCA
TCAGGAAGAACCTGGGCGGCCGGCCTAAGTCCAATAATCCTAAGGTCGCCGTGAGCATTCGGCTCGATCCAGAAGTCGTC
GATGCTTTCAAGGCCAAAGGCGAAGGCTGGCAAAGCCGGATAAATGAGACACTGCGGAAGGCGGTAGGGCTATAG

Structures

Toxin

• AlphaFold DB: C6B7E2

Antitoxin

• AlphaFold DB: C6B7E3