Detailed information of TA system TA023907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 74045..74601
• Replicon: plasmid pR132505
• Accession: NC_012854
• Organism: Rhizobium leguminosarum bv. trifolii WSM1325

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: RLEG_RS36990
• Protein ID: WP_012760638.1
• Coordinates: 74311..74601 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C6BAK2
• Locus tag: RLEG_RS34420
• Protein ID: WP_012760637.1
• Coordinates: 74045..74323 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG_RS34400 (Rleg_6356)	69318..70205	-	888	WP_003555441.1	sugar ABC transporter permease	-
RLEG_RS34405 (Rleg_6357)	70308..71540	-	1233	WP_012760634.1	ABC transporter substrate-binding protein	-
RLEG_RS34410 (Rleg_6358)	71599..72549	-	951	WP_012760635.1	sugar phosphate isomerase/epimerase	-
RLEG_RS34415 (Rleg_6359)	72772..73842	+	1071	WP_012760636.1	LacI family transcriptional regulator	-
RLEG_RS34420 (Rleg_6360)	74045..74323	+	279	WP_012760637.1	CopG family ribbon-helix-helix protein	Antitoxin
RLEG_RS36990 (Rleg_6361)	74311..74601	+	291	WP_012760638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RLEG_RS34430 (Rleg_6362)	74766..75209	+	444	WP_003555446.1	acetyl-CoA carboxylase biotin carboxyl carrier protein subunit	-
RLEG_RS34435 (Rleg_6363)	75209..76594	+	1386	WP_012760639.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
RLEG_RS34440 (Rleg_6364)	76591..77028	+	438	WP_003555448.1	hypothetical protein	-
RLEG_RS34445 (Rleg_6365)	77025..77732	+	708	WP_012760640.1	5-oxoprolinase subunit PxpB	-
RLEG_RS34450 (Rleg_6366)	77729..78724	+	996	WP_012760641.1	biotin-dependent carboxyltransferase family protein	-
RLEG_RS34455 (Rleg_6367)	78762..79529	+	768	WP_003555453.1	LamB/YcsF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..294782	294782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10982.76 Da
• Isoelectric Point: 9.6206

>T23907 WP_012760638.1 NC_012854:74311-74601 [Rhizobium leguminosarum bv. trifolii WSM1325]
MPQVVFSPAAIRDLERLREFLRLKNPSAAKRAGETILKGLRALGVHPHIGRLIEDLPEQYREWLIDFGDSGYVARYRIDG
DILMILAVRHQKEAGF

Nucleotide

• Length: 291 bp

>T23907 NC_012854:74311-74601 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGCCACAAGTAGTTTTCTCCCCGGCCGCAATCCGCGACCTCGAGCGGCTCCGAGAGTTCTTACGACTGAAAAACCCATC
GGCCGCCAAGCGAGCAGGCGAAACTATACTCAAAGGCTTGAGAGCCCTCGGCGTACATCCCCATATTGGACGCCTCATCG
AGGACTTGCCCGAGCAATACCGGGAGTGGCTCATCGATTTTGGAGATAGCGGATACGTCGCTCGTTATCGTATTGATGGC
GATATTTTGATGATCCTCGCGGTACGGCATCAGAAGGAAGCAGGGTTCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10634.84 Da
• Isoelectric Point: 5.2076

>AT23907 WP_012760637.1 NC_012854:74045-74323 [Rhizobium leguminosarum bv. trifolii WSM1325]
MASPTSLKLDDELKGRVQQLAEARRRSSHWIMREAIAQYVEREEKREALRQETLDAWHEFKATGLHVTGAEVESWLSTWG
TDDELSAPECHK

Nucleotide

• Length: 279 bp

>AT23907 NC_012854:74045-74323 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGGCATCGCCGACCTCGCTCAAACTCGATGATGAGCTAAAGGGCCGCGTTCAGCAACTGGCCGAGGCTCGCCGCCGCTC
GTCCCACTGGATCATGCGTGAAGCTATTGCGCAATATGTTGAGCGCGAGGAGAAGCGTGAGGCGCTGCGACAGGAAACGC
TTGACGCCTGGCATGAATTTAAAGCGACGGGACTGCATGTCACCGGCGCCGAGGTCGAAAGCTGGCTTTCGACTTGGGGA
ACTGACGACGAGTTGTCCGCACCCGAATGCCACAAGTAG

Structures

Antitoxin

• AlphaFold DB: C6BAK2