Detailed information of TA system TA239057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 43895..44496
• Replicon: plasmid pGA_Eco1
• Accession: NZ_CP093921
• Organism: Escherichia coli strain ST167

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: MOO49_RS24140
• Protein ID: WP_168420618.1
• Coordinates: 43895..44275 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: MOO49_RS24145
• Protein ID: WP_001190712.1
• Coordinates: 44275..44496 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO49_RS24110	39917..40945	+	1029	WP_168420624.1	tyrosine-type recombinase/integrase	-
MOO49_RS24115	41055..41363	+	309	WP_000349257.1	hypothetical protein	-
MOO49_RS24120	41360..41836	+	477	WP_000861174.1	hypothetical protein	-
MOO49_RS24125	41833..42327	+	495	WP_000640906.1	dUTP diphosphatase	-
MOO49_RS24130	42342..43004	+	663	WP_168420622.1	DUF2829 domain-containing protein	-
MOO49_RS24135	43011..43811	+	801	WP_168420620.1	hypothetical protein	-
MOO49_RS24140	43895..44275	-	381	WP_168420618.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MOO49_RS24145	44275..44496	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MOO49_RS24150	44569..44958	-	390	WP_168420616.1	S24 family peptidase	-
MOO49_RS24155	45082..45333	-	252	WP_063074807.1	DNA polymerase III subunit theta	-
MOO49_RS24160	45648..46406	-	759	WP_072756859.1	hypothetical protein	-
MOO49_RS24165	46403..46612	-	210	WP_075660655.1	hypothetical protein	-
MOO49_RS24170	46692..46868	-	177	WP_157902266.1	hypothetical protein	-
MOO49_RS24175	46868..47161	-	294	WP_075660654.1	hypothetical protein	-
MOO49_RS24180	47174..47578	-	405	WP_080077662.1	hypothetical protein	-
MOO49_RS24185	47571..47852	-	282	WP_102218741.1	ASCH domain-containing protein	-
MOO49_RS24190	47845..48036	-	192	WP_242806062.1	DUF551 domain-containing protein	-
MOO49_RS24195	48547..48672	-	126	Protein_55	DUF551 domain-containing protein	-
MOO49_RS24200	48669..48878	-	210	WP_000951710.1	hypothetical protein	-
MOO49_RS24205	48880..49074	-	195	WP_000224731.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90296	90296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13605.50 Da
• Isoelectric Point: 5.6406

>T239057 WP_168420618.1 NZ_CP093921:c44275-43895 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Nucleotide

• Length: 381 bp

>T239057 NZ_LR890583:c5189741-5189358 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT239057 WP_001190712.1 NZ_CP093921:c44496-44275 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT239057 NZ_LR890583:c5189983-5189741 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6