Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 69217..69742 Replicon plasmid pGA_EcoNDM5
Accession NZ_CP093920
Organism Escherichia coli strain ST167

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MOO49_RS23685 Protein ID WP_001159871.1
Coordinates 69437..69742 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag MOO49_RS23680 Protein ID WP_000813630.1
Coordinates 69217..69435 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO49_RS23655 (64416) 64416..65113 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
MOO49_RS23660 (65367) 65367..66389 - 1023 WP_000361402.1 helicase UvrD -
MOO49_RS23665 (66374) 66374..67939 - 1566 WP_001128474.1 AAA family ATPase -
MOO49_RS23670 (68014) 68014..68430 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
MOO49_RS23675 (68427) 68427..68657 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
MOO49_RS23680 (69217) 69217..69435 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MOO49_RS23685 (69437) 69437..69742 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MOO49_RS23690 (69743) 69743..70549 + 807 WP_000016970.1 site-specific integrase -
MOO49_RS23695 (71271) 71271..72026 + 756 WP_000852146.1 replication initiation protein RepE -
MOO49_RS23700 (72614) 72614..73780 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IIa / mph(A) / sitABCD iucA / iucB / iucC / iucD / iutA 1..100291 100291
- flank IS/Tn - - 64736..65113 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T239052 WP_001159871.1 NZ_CP093920:69437-69742 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T239052 NZ_LR890583:c3872971-3872654 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAAATGCGCAATGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT239052 WP_000813630.1 NZ_CP093920:69217-69435 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT239052 NZ_LR890583:c3872661-3872374 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References