Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4346928..4347425 Replicon chromosome
Accession NZ_CP093919
Organism Escherichia coli strain ST167

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MOO49_RS20975 Protein ID WP_001173343.1
Coordinates 4346928..4347101 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MOO49_RS20980 Protein ID WP_000398619.1
Coordinates 4347129..4347425 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO49_RS20965 (4343598) 4343598..4345055 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
MOO49_RS20970 (4345292) 4345292..4346809 + 1518 WP_001295074.1 lysine--tRNA ligase -
MOO49_RS20975 (4346928) 4346928..4347101 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MOO49_RS20980 (4347129) 4347129..4347425 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MOO49_RS20985 (4347652) 4347652..4347924 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
MOO49_RS20990 (4347936) 4347936..4348166 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MOO49_RS20995 (4348347) 4348347..4349978 + 1632 WP_001216477.1 sensor histidine kinase -
MOO49_RS21000 (4349975) 4349975..4350694 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T239050 WP_001173343.1 NZ_CP093919:c4347101-4346928 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T239050 NZ_LR890583:1896513-1896615 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT239050 WP_000398619.1 NZ_CP093919:c4347425-4347129 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT239050 NZ_LR890583:c1896622-1896477 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References