Detailed information of TA system TA239026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 27691..28216
• Replicon: plasmid pSD21_mcr8
• Accession: NZ_CP093916
• Organism: Enterobacter cloacae strain SD21

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: MQH03_RS23985
• Protein ID: WP_004197633.1
• Coordinates: 27911..28216 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: MQH03_RS23980
• Protein ID: WP_004197642.1
• Coordinates: 27691..27909 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQH03_RS23960 (MQH03_23960)	22956..23936	+	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
MQH03_RS23965 (MQH03_23965)	24378..25169	-	792	WP_221928726.1	ribbon-helix-helix domain-containing protein	-
MQH03_RS23970 (MQH03_23970)	25352..26380	-	1029	WP_032445668.1	Abi family protein	-
MQH03_RS23975 (MQH03_23975)	26541..27122	-	582	WP_072310991.1	hypothetical protein	-
MQH03_RS23980 (MQH03_23980)	27691..27909	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MQH03_RS23985 (MQH03_23985)	27911..28216	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
MQH03_RS23990 (MQH03_23990)	28385..28780	+	396	WP_164530166.1	hypothetical protein	-
MQH03_RS23995 (MQH03_23995)	28807..29130	+	324	WP_004197641.1	hypothetical protein	-
MQH03_RS24000 (MQH03_24000)	29127..30143	+	1017	WP_164530167.1	hypothetical protein	-
MQH03_RS24005 (MQH03_24005)	30341..31126	+	786	WP_046664219.1	site-specific integrase	-
MQH03_RS24010 (MQH03_24010)	31575..32330	+	756	WP_001568031.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-8	-	1..100852	100852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T239026 WP_004197633.1 NZ_CP093916:27911-28216 [Enterobacter cloacae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T239026 NZ_LR890569:c2611722-2610400 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT239026 WP_004197642.1 NZ_CP093916:27691-27909 [Enterobacter cloacae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT239026 NZ_LR890569:c2611988-2611722 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5