Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 86542..88165 Replicon plasmid pSD21_266kb
Accession NZ_CP093915
Organism Enterobacter cloacae strain SD21

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag MQH03_RS22820 Protein ID WP_000088046.1
Coordinates 86840..88165 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag MQH03_RS22815 Protein ID WP_024136327.1
Coordinates 86542..86835 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQH03_RS22790 (MQH03_22790) 82414..83655 + 1242 WP_001053338.1 VWA domain-containing protein -
MQH03_RS24815 83746..83877 + 132 WP_000374059.1 hypothetical protein -
MQH03_RS22795 (MQH03_22795) 84218..84499 - 282 WP_000077926.1 hypothetical protein -
MQH03_RS22800 (MQH03_22800) 84549..84740 - 192 WP_000398480.1 hypothetical protein -
MQH03_RS22805 (MQH03_22805) 84832..85173 - 342 WP_001151575.1 hypothetical protein -
MQH03_RS22810 (MQH03_22810) 85507..85938 + 432 WP_011152969.1 hypothetical protein -
MQH03_RS22815 (MQH03_22815) 86542..86835 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
MQH03_RS22820 (MQH03_22820) 86840..88165 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
MQH03_RS22825 (MQH03_22825) 88226..88432 + 207 WP_000134171.1 hypothetical protein -
MQH03_RS22830 (MQH03_22830) 88534..88944 + 411 WP_000985911.1 hypothetical protein -
MQH03_RS22835 (MQH03_22835) 88957..89772 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
MQH03_RS22840 (MQH03_22840) 90026..90451 + 426 WP_001043843.1 hypothetical protein -
MQH03_RS22845 (MQH03_22845) 91000..91308 + 309 WP_001224687.1 hypothetical protein -
MQH03_RS22850 (MQH03_22850) 91324..92181 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
MQH03_RS22855 (MQH03_22855) 92243..92446 + 204 WP_001194555.1 hypothetical protein -
MQH03_RS22860 (MQH03_22860) 92788..93144 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaCTX-M-55 / blaTEM-1B / rmtB / fosA3 / aac(3)-IId / ant(3'')-Ia / lnu(F) / sul3 / tet(A) / aph(6)-Id / floR / aph(3')-Ia / mph(A) / ARR-3 / dfrA14 - 1..266158 266158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T239025 WP_000088046.1 NZ_CP093915:86840-88165 [Enterobacter cloacae]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T239025 NZ_LR890569:1863603-1863705 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT239025 WP_024136327.1 NZ_CP093915:86542-86835 [Enterobacter cloacae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT239025 NZ_LR890569:c1863712-1863567 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References