Detailed information of TA system TA023902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 169728..170418
• Replicon: chromosome
• Accession: NC_012850
• Organism: Rhizobium leguminosarum bv. trifolii WSM1325

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: RLEG_RS00870
• Protein ID: WP_012755903.1
• Coordinates: 170053..170418 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: C6AZF6
• Locus tag: RLEG_RS00865
• Protein ID: WP_012755902.1
• Coordinates: 169728..170051 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG_RS00840	165149..165349	-	201	WP_017995941.1	hypothetical protein	-
RLEG_RS00845	165675..166520	+	846	WP_012755898.1	methyltransferase domain-containing protein	-
RLEG_RS00850	166709..167284	-	576	WP_012755899.1	chloramphenicol phosphotransferase	-
RLEG_RS00855	167359..168849	-	1491	WP_012755900.1	MFS transporter	-
RLEG_RS00860	169109..169504	-	396	WP_012755901.1	hypothetical protein	-
RLEG_RS00865	169728..170051	-	324	WP_012755902.1	putative addiction module antidote protein	Antitoxin
RLEG_RS00870	170053..170418	-	366	WP_012755903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RLEG_RS00875	170415..170888	-	474	WP_012755904.1	hypothetical protein	-
RLEG_RS00885	171262..172221	-	960	WP_012755905.1	nitronate monooxygenase family protein	-
RLEG_RS00890	172508..173692	+	1185	WP_012755906.1	hypothetical protein	-
RLEG_RS00895	173746..174627	-	882	WP_012755907.1	polyphosphate kinase 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (30-120)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13758.64 Da
• Isoelectric Point: 10.0179

>T23902 WP_012755903.1 NC_012850:c170418-170053 [Rhizobium leguminosarum bv. trifolii WSM1325]
MTGGPSQHSGQQKVDYSREGVNYSSRKYTVLQTAEFQKWLRKLKDRNARDRIVTRIVRAEAGNLGDHKFFDGIGELRLDY
GPGYRLYFAMQGSVVVILLCGGDKGSQERDIKRAIEMSKEI

Nucleotide

• Length: 366 bp

>T23902 NC_012850:c170418-170053 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGACAGGAGGGCCGTCTCAGCACTCCGGTCAACAAAAAGTTGACTACTCGCGGGAGGGTGTTAATTATAGTTCACGGAA
ATACACGGTTCTCCAGACAGCCGAATTCCAGAAATGGCTGCGCAAGCTGAAGGATCGGAATGCAAGAGACCGAATTGTCA
CGCGCATCGTTCGTGCGGAGGCTGGCAATTTGGGCGACCACAAATTCTTCGATGGCATCGGGGAATTGAGGCTCGACTAC
GGTCCTGGGTATCGTTTGTATTTTGCAATGCAGGGCAGCGTTGTCGTTATTCTCCTGTGCGGGGGCGACAAGGGCAGCCA
GGAGCGGGACATCAAGCGAGCAATCGAAATGTCCAAGGAGATATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11415.73 Da
• Isoelectric Point: 4.4698

>AT23902 WP_012755902.1 NC_012850:c170051-169728 [Rhizobium leguminosarum bv. trifolii WSM1325]
MALEIIPFDATEFLDTEESQAHLIEDAFESGDAGYIAHALGVVAKARGMSQVARDAGVTREALYKSLSEKGDPKLSTLIG
VAAALGYKLTATRVGDSDTREDERRSA

Nucleotide

• Length: 324 bp

>AT23902 NC_012850:c170051-169728 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGGCGCTTGAAATCATCCCTTTTGACGCAACCGAGTTTCTGGACACCGAAGAATCTCAGGCCCATCTCATTGAAGACGC
GTTTGAAAGCGGTGATGCAGGTTATATCGCGCACGCATTGGGCGTTGTTGCGAAAGCTCGTGGCATGTCACAAGTCGCCC
GGGATGCCGGCGTTACTCGCGAGGCTCTGTACAAATCGCTCAGTGAAAAAGGCGACCCAAAACTCTCGACGCTCATCGGC
GTAGCTGCAGCTCTCGGATACAAGCTGACAGCGACGCGTGTGGGTGATTCAGATACGCGCGAGGACGAGCGCCGATCTGC
TTAG

Structures

Antitoxin

• AlphaFold DB: C6AZF6