Detailed information of TA system TA239019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2962584..2963174
• Replicon: chromosome
• Accession: NZ_CP093914
• Organism: Enterobacter cloacae strain SD21

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: MQH03_RS13970
• Protein ID: WP_061771414.1
• Coordinates: 2962584..2962916 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A427KRT2
• Locus tag: MQH03_RS13975
• Protein ID: WP_020687575.1
• Coordinates: 2962917..2963174 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQH03_RS13930 (MQH03_13930)	2958256..2958735	+	480	WP_001588075.1	DUF4756 family protein	-
MQH03_RS13935 (MQH03_13935)	2958894..2959298	+	405	WP_001588074.1	H-NS family nucleoid-associated regulatory protein	-
MQH03_RS13940 (MQH03_13940)	2959424..2959804	-	381	WP_004151487.1	hypothetical protein	-
MQH03_RS13945 (MQH03_13945)	2959857..2960147	-	291	WP_002911787.1	hypothetical protein	-
MQH03_RS13950 (MQH03_13950)	2960226..2960537	-	312	WP_186599245.1	hypothetical protein	-
MQH03_RS13955 (MQH03_13955)	2960551..2960880	-	330	WP_001588070.1	hypothetical protein	-
MQH03_RS13960 (MQH03_13960)	2960896..2961114	-	219	WP_002911792.1	AlpA family transcriptional regulator	-
MQH03_RS13965 (MQH03_13965)	2961262..2962125	-	864	WP_186599244.1	DUF6387 family protein	-
MQH03_RS13970 (MQH03_13970)	2962584..2962916	-	333	WP_061771414.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MQH03_RS13975 (MQH03_13975)	2962917..2963174	-	258	WP_020687575.1	antitoxin	Antitoxin
MQH03_RS13980 (MQH03_13980)	2963510..2963899	-	390	WP_061771413.1	Rid family hydrolase	-
MQH03_RS13985 (MQH03_13985)	2964041..2964973	+	933	WP_061771412.1	LysR family transcriptional regulator	-
MQH03_RS13990 (MQH03_13990)	2965021..2965971	-	951	WP_013097829.1	HTH-type transcriptional regulator Cbl	-
MQH03_RS13995 (MQH03_13995)	2966068..2966985	-	918	WP_010432103.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-85)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11847.81 Da
• Isoelectric Point: 10.5834

>T239019 WP_061771414.1 NZ_CP093914:c2962916-2962584 [Enterobacter cloacae]
MDRGEIWLVSLDPIASHEQSGKRPVLIVSKASFNKVIRLPVVVPVTSGGNFARIAGFTVSLDGAGTKTMGVIRCDQPRTI
DIVARNGKRLERVPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T239019 NZ_LR890564:c4691225-4690941 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9437.90 Da
• Isoelectric Point: 4.3556

>AT239019 WP_020687575.1 NZ_CP093914:c2963174-2962917 [Enterobacter cloacae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSIDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAT
GKEIL

Nucleotide

• Length: 258 bp

>AT239019 NZ_LR890564:c4691457-4691215 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A427KRT2