Detailed information of TA system TA023901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 704430..704977
• Replicon: plasmid pR132501
• Accession: NC_012848
• Organism: Rhizobium leguminosarum bv. trifolii WSM1325

Toxin (Protein)

• Gene name: parE
• Uniprot ID: C6B5Z4
• Locus tag: RLEG_RS26250
• Protein ID: WP_012755607.1
• Coordinates: 704430..704723 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: C6B5Z5
• Locus tag: RLEG_RS26255
• Protein ID: WP_012755608.1
• Coordinates: 704723..704977 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG_RS26225	699962..701083	+	1122	WP_012755602.1	ABC transporter permease	-
RLEG_RS26230	701086..701886	+	801	WP_012755603.1	sugar ABC transporter ATP-binding protein	-
RLEG_RS26235	701908..702705	+	798	WP_012755604.1	TIM barrel protein	-
RLEG_RS26240	702735..703757	+	1023	WP_012755605.1	Gfo/Idh/MocA family oxidoreductase	-
RLEG_RS26245	703790..704116	+	327	WP_012755606.1	RpiR family transcriptional regulator	-
RLEG_RS26250	704430..704723	-	294	WP_012755607.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RLEG_RS26255	704723..704977	-	255	WP_012755608.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
RLEG_RS26260	705113..705309	-	197	Protein_649	helix-turn-helix transcriptional regulator	-
RLEG_RS26265	705453..706637	+	1185	WP_012755610.1	MFS transporter	-
RLEG_RS38210	707081..707257	+	177	WP_003549623.1	hypothetical protein	-
RLEG_RS26270	707292..708344	+	1053	WP_012755611.1	ribosome small subunit-dependent GTPase A	-
RLEG_RS26280	709170..709825	+	656	Protein_653	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..828924	828924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Antitoxin ParD (3-70)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11215.73 Da
• Isoelectric Point: 5.8988

>T23901 WP_012755607.1 NC_012848:c704723-704430 [Rhizobium leguminosarum bv. trifolii WSM1325]
MRFSLSVEAEEDIIAIAEQSVRIFGSAQARRYHDELFAVLDLIAANPQMAREREEIWPHVRIHPFKAHLVVYRIEENGAI
FVIRIRHGHEDWAGDPG

Nucleotide

• Length: 294 bp

>T23901 NC_012848:c704723-704430 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGCGTTTTAGCCTTTCTGTCGAAGCGGAAGAGGACATCATTGCAATCGCCGAGCAAAGTGTCCGAATTTTCGGATCTGC
ACAGGCTAGAAGGTATCATGACGAGCTGTTCGCGGTGCTTGACCTGATTGCCGCGAATCCGCAAATGGCGCGTGAACGAG
AAGAGATATGGCCGCACGTTAGGATCCATCCGTTCAAAGCGCATCTCGTTGTCTACCGGATCGAAGAGAACGGAGCCATT
TTCGTGATCCGAATACGCCATGGACATGAAGACTGGGCCGGCGATCCTGGTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9279.39 Da
• Isoelectric Point: 10.2106

>AT23901 WP_012755608.1 NC_012848:c704977-704723 [Rhizobium leguminosarum bv. trifolii WSM1325]
MATMNVSLPDPMKDWVEAQARTGRYSNASDYVRDLIRRDQARSDKIAAMQRFVDDGVKSGVGGRSKDELFAAAVARAETT
RGSR

Nucleotide

• Length: 255 bp

>AT23901 NC_012848:c704977-704723 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGGCTACTATGAACGTATCTTTACCAGATCCAATGAAAGATTGGGTCGAGGCGCAAGCGAGGACCGGGCGATATTCCAA
TGCCAGTGACTATGTGCGCGACCTGATTCGCAGGGACCAAGCACGCAGTGATAAGATTGCTGCCATGCAACGCTTTGTCG
ACGACGGTGTCAAAAGTGGTGTTGGCGGTCGCTCGAAGGACGAACTCTTTGCAGCGGCAGTCGCGCGCGCGGAAACGACC
CGCGGCAGCAGATAA

Structures

Toxin

• AlphaFold DB: C6B5Z4

Antitoxin

• AlphaFold DB: C6B5Z5