Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 596039..596615 Replicon chromosome
Accession NZ_CP093914
Organism Enterobacter cloacae strain SD21

Toxin (Protein)


Gene name shpA Uniprot ID A0A5E1APA0
Locus tag MQH03_RS02835 Protein ID WP_023620650.1
Coordinates 596039..596326 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A5E1AP18
Locus tag MQH03_RS02840 Protein ID WP_023620649.1
Coordinates 596313..596615 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQH03_RS02810 (MQH03_02810) 591103..591798 + 696 WP_248766758.1 winged helix-turn-helix domain-containing protein -
MQH03_RS02815 (MQH03_02815) 592001..592471 + 471 WP_013095432.1 MarR family transcriptional regulator -
MQH03_RS02820 (MQH03_02820) 592468..593535 + 1068 WP_029882194.1 HlyD family secretion protein -
MQH03_RS02825 (MQH03_02825) 593525..594601 + 1077 WP_038418624.1 DUF2955 domain-containing protein -
MQH03_RS02830 (MQH03_02830) 594571..595869 - 1299 WP_038418625.1 DUF445 domain-containing protein -
MQH03_RS02835 (MQH03_02835) 596039..596326 + 288 WP_023620650.1 BrnT family toxin Toxin
MQH03_RS02840 (MQH03_02840) 596313..596615 + 303 WP_023620649.1 BrnA antitoxin family protein Antitoxin
MQH03_RS02845 (MQH03_02845) 596646..597284 - 639 WP_038418626.1 LysE family translocator -
MQH03_RS02850 (MQH03_02850) 597333..598076 - 744 WP_038418627.1 AraC family transcriptional regulator -
MQH03_RS02855 (MQH03_02855) 598213..599583 + 1371 WP_248766759.1 NAD-dependent succinate-semialdehyde dehydrogenase -
MQH03_RS02860 (MQH03_02860) 599625..599945 - 321 WP_013095439.1 hypothetical protein -
MQH03_RS02865 (MQH03_02865) 599945..600490 - 546 WP_013095440.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11246.60 Da        Isoelectric Point: 6.8813

>T239009 WP_023620650.1 NZ_CP093914:596039-596326 [Enterobacter cloacae]
MPTEYEWDSDKAKSNQQKHGIRFEDAVLVFDDPYHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGGEIIRIIS
ARKADRKERNRYEHR

Download         Length: 288 bp

>T239009 NZ_LR890563:c140536-140231 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11414.05 Da        Isoelectric Point: 10.3694

>AT239009 WP_023620649.1 NZ_CP093914:596313-596615 [Enterobacter cloacae]
MSIVKHKRGSSPEVSARRDAELKALAEKPEAEIDYSDIPATDDKQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT239009 NZ_LR890563:c140756-140538 [Klebsiella pneumoniae]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1APA0

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP18

References