Detailed information of TA system TA023899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 448358..448893
• Replicon: plasmid pR132501
• Accession: NC_012848
• Organism: Rhizobium leguminosarum bv. trifolii WSM1325

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C6B5C2
• Locus tag: RLEG_RS25120
• Protein ID: WP_012755427.1
• Coordinates: 448618..448893 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C6B5C1
• Locus tag: RLEG_RS25115
• Protein ID: WP_012755426.1
• Coordinates: 448358..448618 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG_RS25095	444132..445013	-	882	WP_012755422.1	ABC transporter permease	-
RLEG_RS25100	445025..445843	-	819	WP_012755423.1	ABC transporter ATP-binding protein	-
RLEG_RS25105	445940..446953	-	1014	WP_012755424.1	ABC transporter substrate-binding protein	-
RLEG_RS25110	447326..448135	-	810	WP_050786654.1	FadR family transcriptional regulator	-
RLEG_RS25115	448358..448618	+	261	WP_012755426.1	ribbon-helix-helix domain-containing protein	Antitoxin
RLEG_RS25120	448618..448893	+	276	WP_012755427.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RLEG_RS25125	448900..449478	-	579	Protein_413	substrate-binding domain-containing protein	-
RLEG_RS25130	449589..450518	+	930	WP_012755429.1	substrate-binding domain-containing protein	-
RLEG_RS25135	450535..452679	+	2145	WP_012755430.1	methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..828924	828924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10930.60 Da
• Isoelectric Point: 9.9696

>T23899 WP_012755427.1 NC_012848:448618-448893 [Rhizobium leguminosarum bv. trifolii WSM1325]
MEIKWTSKAVSDVSRLYDFLTPVDRQAAVRTVRALTAAPARLLEQPRLGERLEEFDPREVRRILVGRYELRYEIQQSMIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T23899 NC_012848:448618-448893 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGGAGATCAAGTGGACGAGCAAAGCGGTCTCGGACGTCTCCCGCTTGTACGACTTTCTCACTCCGGTCGACCGGCAAGC
GGCGGTCCGAACCGTGCGGGCACTGACGGCAGCGCCTGCACGGCTGCTTGAGCAGCCGCGCCTCGGCGAGCGGCTCGAGG
AGTTCGATCCTCGAGAGGTCCGCCGTATTCTCGTCGGCCGTTACGAGCTGCGCTATGAAATCCAGCAGTCGATGATCTAT
GTCTTGCGCCTTTGGCATACGCGTGAGGACAGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9787.06 Da
• Isoelectric Point: 4.4964

>AT23899 WP_012755426.1 NC_012848:448358-448618 [Rhizobium leguminosarum bv. trifolii WSM1325]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALMAWIDQEEERRRLTLEALADVDDGNVIEHQSVQAWADSLDSDE
PISLPR

Nucleotide

• Length: 261 bp

>AT23899 NC_012848:448358-448618 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGGAGACCAAGGTTCTGACTGCTCATGTTCCGCTACCGCTTGCGCAAAAGGTCGATCAACTCGCGTCGCGGCTCGAACG
TTCGCGTGGCTGGATCGTCAAGCAGGCTCTCATGGCATGGATCGACCAGGAGGAGGAGCGCCGGCGTTTGACGCTCGAGG
CGTTGGCCGACGTCGATGATGGTAACGTTATCGAGCACCAGTCTGTTCAAGCCTGGGCCGATAGCCTCGATAGCGACGAG
CCGATCTCTTTGCCTCGGTGA

Structures

Toxin

• AlphaFold DB: C6B5C2

Antitoxin

• AlphaFold DB: C6B5C1