Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 39576..40252 Replicon plasmid pR132501
Accession NC_012848
Organism Rhizobium leguminosarum bv. trifolii WSM1325

Toxin (Protein)


Gene name vapC Uniprot ID C6B4A0
Locus tag RLEG_RS23210 Protein ID WP_012755158.1
Coordinates 39576..40001 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6N9Z9K0
Locus tag RLEG_RS23215 Protein ID WP_027664431.1
Coordinates 39998..40252 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RLEG_RS23195 35708..36278 + 571 Protein_29 GNAT family N-acetyltransferase -
RLEG_RS23200 36362..37144 - 783 WP_012755156.1 siderophore-iron reductase FhuF -
RLEG_RS23205 37154..39337 - 2184 WP_012755157.1 TonB-dependent siderophore receptor -
RLEG_RS23210 39576..40001 - 426 WP_012755158.1 type II toxin-antitoxin system VapC family toxin Toxin
RLEG_RS23215 39998..40252 - 255 WP_027664431.1 plasmid stabilization protein Antitoxin
RLEG_RS23220 40559..41131 - 573 WP_012755160.1 TetR/AcrR family transcriptional regulator -
RLEG_RS23225 41270..41539 + 270 Protein_35 glutathione S-transferase C-terminal domain-containing protein -
RLEG_RS23230 41552..41863 + 312 Protein_36 peptidoglycan-binding protein -
RLEG_RS23235 42211..43664 + 1454 Protein_37 putative DNA binding domain-containing protein -
RLEG_RS23240 43925..45142 + 1218 WP_012755163.1 GGDEF domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..828924 828924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15264.57 Da        Isoelectric Point: 4.8411

>T23898 WP_012755158.1 NC_012848:c40001-39576 [Rhizobium leguminosarum bv. trifolii WSM1325]
MILIDTNVISEPWKPTPDSQVLAWIDAQAVETLFVSAITVAELRFGIAAMPSGRRKTVLYDRLEEEVLPLFSGRVLSFDM
AVSKSYSELMARARVAGRAIGKADAYIAATAAANGFIVATRDTSPFEAAGLNVINPWYAPT

Download         Length: 426 bp

>T23898 NC_012848:c40001-39576 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGATCCTGATCGATACCAACGTCATCTCGGAGCCATGGAAGCCCACACCCGATAGTCAGGTGCTGGCGTGGATCGATGC
TCAAGCAGTGGAAACACTGTTCGTCTCGGCGATTACAGTAGCTGAGTTACGGTTCGGCATCGCCGCGATGCCTAGCGGAA
GACGAAAGACCGTCCTCTACGATCGACTAGAAGAGGAAGTTCTACCTCTATTTTCTGGACGCGTTCTATCGTTCGACATG
GCTGTGTCAAAGAGCTATTCGGAATTAATGGCGCGAGCACGCGTAGCAGGTCGGGCAATCGGTAAAGCAGATGCCTATAT
CGCCGCAACTGCCGCGGCGAACGGATTTATTGTCGCTACCCGCGACACTAGTCCCTTCGAAGCCGCAGGACTTAACGTTA
TCAACCCGTGGTACGCACCGACATAG

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9232.44 Da        Isoelectric Point: 6.2235

>AT23898 WP_027664431.1 NC_012848:c40252-39998 [Rhizobium leguminosarum bv. trifolii WSM1325]
MAAVTIRNLSEETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNNDVDNLQLVRDKAPAEP
MSFE

Download         Length: 255 bp

>AT23898 NC_012848:c40252-39998 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGGCAGCAGTTACAATACGCAATTTGTCTGAGGAGACACATCGTGCCTTGAGAGTGAGGGCAGCTCATCACGGCCGCAG
TACCGAAGCAGAAATCCGTGACATCCTAGAAACAGCGGTACGGCCAGCTGAGCGCGTCATGTTGGGCTCGTTGCTAGCAT
CGATCGGGCGCGAAGCAGGGCTTTCCAACAACGACGTGGATAATCTGCAGCTCGTCCGAGACAAAGCGCCTGCGGAACCT
ATGAGTTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB C6B4A0

Antitoxin

Source ID Structure
AlphaFold DB A0A6N9Z9K0

References