Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 246939..247464 Replicon plasmid pBio30
Accession NZ_CP093857
Organism Klebsiella pneumoniae strain Bio30

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag MOQ79_RS29410 Protein ID WP_008322213.1
Coordinates 247159..247464 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag MOQ79_RS29405 Protein ID WP_032934863.1
Coordinates 246939..247157 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOQ79_RS29380 (241979) 241979..242536 + 558 WP_013214031.1 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB -
MOQ79_RS29385 (242582) 242582..243025 + 444 WP_101455614.1 F-type conjugal transfer protein TrbF -
MOQ79_RS29390 (243003) 243003..244382 + 1380 WP_072198320.1 conjugal transfer pilus assembly protein TraH -
MOQ79_RS29395 (244382) 244382..246013 + 1632 Protein_300 conjugal transfer mating-pair stabilization protein TraG -
MOQ79_RS29400 (246015) 246015..246077 + 63 Protein_301 IS1 family transposase -
MOQ79_RS29405 (246939) 246939..247157 + 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MOQ79_RS29410 (247159) 247159..247464 + 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
MOQ79_RS29415 (247466) 247466..247780 + 315 WP_008322211.1 hypothetical protein -
MOQ79_RS29420 (247770) 247770..248561 + 792 WP_008322208.1 site-specific integrase -
MOQ79_RS29425 (248760) 248760..250100 - 1341 Protein_306 GntP family transporter -
MOQ79_RS29430 (250198) 250198..250572 + 375 Protein_307 type-F conjugative transfer system pilin assembly protein TrbC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / rmtC / blaNDM-1 htpB 1..316167 316167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T238970 WP_008322213.1 NZ_CP093857:247159-247464 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T238970 NZ_LR890548:c14266-13916 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT238970 WP_032934863.1 NZ_CP093857:246939-247157 [Klebsiella pneumoniae]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT238970 NZ_LR890548:c13911-13609 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References