Detailed information of TA system TA238969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 187653..188178
• Replicon: plasmid pBio30
• Accession: NZ_CP093857
• Organism: Klebsiella pneumoniae strain Bio30

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MOQ79_RS29045
• Protein ID: WP_114502755.1
• Coordinates: 187653..187958 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: MOQ79_RS29050
• Protein ID: WP_004197642.1
• Coordinates: 187960..188178 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ79_RS29035 (183050)	183050..183742	+	693	WP_242809242.1	hypothetical protein	-
MOQ79_RS29040 (183801)	183801..187406	+	3606	WP_242809243.1	phage tail tape measure protein	-
MOQ79_RS29045 (187653)	187653..187958	-	306	WP_114502755.1	type II toxin-antitoxin system toxin CcdB	Toxin
MOQ79_RS29050 (187960)	187960..188178	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MOQ79_RS29055 (188774)	188774..189043	+	270	WP_073558205.1	hypothetical protein	-
MOQ79_RS29060 (189031)	189031..189606	+	576	WP_073558204.1	hypothetical protein	-
MOQ79_RS29065 (189637)	189637..190131	+	495	WP_117264656.1	DNA-binding protein	-
MOQ79_RS29070 (190175)	190175..190543	+	369	WP_117046016.1	hypothetical protein	-
MOQ79_RS29075 (190577)	190577..190780	+	204	WP_004213596.1	HHA domain-containing protein	-
MOQ79_RS29080 (190794)	190794..191024	+	231	WP_049118762.1	hypothetical protein	-
MOQ79_RS29085 (191141)	191141..191395	+	255	WP_025862265.1	hypothetical protein	-
MOQ79_RS29090 (191531)	191531..192307	-	777	WP_110133704.1	DUF2971 domain-containing protein	-
MOQ79_RS29095 (192548)	192548..192871	-	324	WP_110133703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / rmtC / blaNDM-1	htpB	1..316167	316167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11631.30 Da
• Isoelectric Point: 6.4661

>T238969 WP_114502755.1 NZ_CP093857:c187958-187653 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASTRLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T238969 NZ_LR890547:c5219596-5219213 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT238969 WP_004197642.1 NZ_CP093857:c188178-187960 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT238969 NZ_LR890547:c5219838-5219596 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA